FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618487
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC25740.41617689619992015No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21500.3476225045958929No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC20880.3375980416726625No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGG16780.271307238470655No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16700.2700137593837866No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16380.2648398430363128No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT16130.2607977208898489No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15870.2565939138575265No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15070.24365912298884213No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14600.23605993335349004No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14580.23573656358177295No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14530.23492813915248018No Hit
CCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGT14320.23153275654945052No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA14050.22716726463126954No Hit
GCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC13400.21665774705046348No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG13260.2143941586484437No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12900.20857350275753575No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGT12900.20857350275753575No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGCCGTTC12340.19951914914945668No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA12170.1967705060898612No Hit
CCCTTGAGGGCCGCTGATTATTACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTTA11940.19305175371511446No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11100.17947022330299586No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11100.17947022330299586No Hit
CCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT10900.17623652558582475No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10880.17591315581410766No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10870.1757514709282491No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10850.17542810115653198No Hit
GCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAA10560.17073923946663389No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA10050.1624933102878476No Hit
CCCAATGGCCGTTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC9710.15699602416865674No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9550.15440906599491985No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG9400.15198379270704154No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9380.15166042293532442No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9320.1506903136201731No Hit
GTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTTACAGTATTTCTT9320.1506903136201731No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9240.14939683453330466No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9230.1492351496474461No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9190.1485884101040119No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8880.1435761786423967No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8750.14147427512623548No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG8670.14018079603936703No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8640.13969574138179136No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8580.13872563206664004No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC8530.13791720763734727No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC8460.13678541343633738No Hit
GTGCAGCATGGGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC8450.13662372855047883No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT8330.1346835099201762No Hit
GTAATAAACAGCCGTGTCCTCGGGAGTCACAGAGTTCAACTGCAGGGAGA8330.1346835099201762No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8320.13452182503431762No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC8240.13322834594744917No Hit
GTAATAATCAGCGGCCCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAG8110.13112644243128796No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8080.1306413877737123No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG8060.1303180180019952No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA7900.12773105982825833No Hit
CACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7890.12756937494239975No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7710.12465904699694577No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC7700.12449736211108722No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7670.12401230745351156No Hit
GGATGACAGCCTGAACGGCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC7530.12174871905149179No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTAAACTGGTACCAGCAGTTCCCAGGAACGGCCCC7400.11964681553533058No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG7350.1188383911060378No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAGCTCCTCAGAGGA7260.1173832271333108No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7240.1170598573615937No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG7210.11657480270401803No Hit
GCCCTCAAGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG7190.11625143293230093No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT7170.11592806316058381No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7070.11431121430199827No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGACTTGAAG6950.11237099567169559No Hit
GATTATTACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGG6950.11237099567169559No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6930.11204762589997849No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6920.11188594101411994No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC6820.11026909215553439No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG6760.10929898284038307No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACT6670.10784381886765607No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG6640.10735876421008042No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGTCAACAAGGCC6580.10638865489492907No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCTTGAGGGCCGCTG6440.10412506649290931No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC6350.10266990252018231No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6320.10218484786260665No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6270.10137642343331388No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGAAATACTGTAAA6220.10056799900402111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1650.048.0253752
GGTATCA4950.043.8598941
GTATCAA12750.043.245611
TATCAAC14600.037.75672
ATCAACG14950.037.1032183
TCAACGC15250.036.1473964
CAACGCA15450.035.679475
AACGCAG15950.034.7798086
ACGGGTA709.818432E-734.45855716
TACTATC400.002612824334.4529882
TGGTTTG3850.034.10508770
ACGCAGA16600.033.628237
AGAGTAC16400.033.62082311
CGCAGAG16550.033.524338
CAGAGTA16600.033.21575510
AATGGTA1401.4319521E-632.8261470
AATAGCG552.9750064E-431.8487562
TATTAGA652.3415245E-531.8027572
GCAGAGT17450.031.7952829
ATGCAAC802.7831393E-630.1536791
Produced by FastQC (version 0.11.5)