FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705813
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTC55830.3272926164825804No Hit
GTATAATACTTATTCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT48790.28602197310021676No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40540.23765793788650924No Hit
GAGTATTACTAACTATTTGGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGT29990.17581059588595No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29700.17411052676934693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28090.1646722120185507No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26710.1565822279464396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25390.1488439823122464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25240.14796463621745173No Hit
CCTTCCACCCTGTCTGCATCTGTAGCAGACAGAGTCACCATCACTTGCCG25220.1478473900714791No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24940.14620594402786238No Hit
GTCTGCATCTGTAGCAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA24350.14274718272166997No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24160.14163334433493002No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23050.13512618323344938No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAATGTC21100.12369468400111852No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20980.12299120712528278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19820.11619093065887058No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19680.1153702076370622No Hit
GTAGCAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTAACTATTTGGCCTGGTATCAACAGAAA19420.1138460077394181No Hit
GGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGTCTAGTTTAGAAAATGGGGTCCCAT18990.11132521560100668No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18950.11109072330906143No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18650.10933203111947207No Hit
GAATTCACTCTCGCCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA18090.1060491390322386No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17800.10434906991563554No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17700.10376283918577241No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17360.1017696547042378No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17160.10059719324451155No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17140.10047994709853894No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17100.10024545480659369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG36900.034.28743
TCAACGC37300.033.7348564
CAACGCA38300.032.944065
AACGCAG39750.031.8290546
ACGCAGA42050.030.0881087
AGAGTAC43300.029.45836411
CGCAGAG43850.028.9316398
GCAGAGT45400.028.019829
AGTACGG36850.026.38192413
GAGTACG37850.025.6849112
TATTAGA2150.025.6552562
ATTAGAG2450.025.328023
CCGTTAA1101.1605607E-625.07291616
GTATCAA53200.024.765681
TTCTGTC23950.023.46584369
TACGGGG19600.023.21803315
TATCAAC56850.023.1647572
TGTTAGA9000.023.0871770
GTACGGG41550.023.06580414
AATCATC21800.022.8134432
Produced by FastQC (version 0.11.5)