FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705813
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTC58050.34030693868554174No Hit
GTATAATACTTATTCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT47420.27799061210109194No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39300.23038867683620656No Hit
GAGTATTACTAACTATTTGGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGT29980.17575197281296367No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26880.15757882018720692No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25360.14866811309328748No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24710.14485761334917718No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24210.1419264596998616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24020.14081262131312167No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23940.14034363672923117No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22870.13407096791969575No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAATGTC22230.1303190912485718No Hit
GTCTGCATCTGTAGCAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21280.12474989931487215No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21040.12334294556320066No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20550.12047041498687136No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20250.118711722797282No Hit
CCTTCCACCCTGTCTGCATCTGTAGCAGACAGAGTCACCATCACTTGCCG20200.11841860743235044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18770.11003550799530781No Hit
GGTATCAACAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGTCTAGTTTAGAAAATGGGGTCCCAT17890.10487667757251234No Hit
GAATTCACTCTCGCCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA17400.10200414699618304No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17240.10106617782840205No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17240.10106617782840205No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17150.10053857017152525No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17130.10042132402555264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC38550.033.670994
CAACGCA38700.033.6294485
ATCAACG38950.033.5019953
AACGCAG39800.032.960486
ACGCAGA42500.030.8755727
AGAGTAC43100.030.3702911
CGCAGAG44200.029.6924048
GCAGAGT45500.029.0711699
AGTACGG38400.026.73176813
GAGTACG38850.026.59946412
TGTGTCG8450.026.10770470
GTATCAA54000.025.380761
GTACGGG43150.023.7085814
TACGGGG20750.023.40620215
TATCAAC58950.023.0702442
GTATAAT18650.022.341991
ATAGGTG6800.022.2783324
CATAGGT6700.022.0969623
ATACTTA18600.022.0285386
ATTCGGC18400.021.72825425
Produced by FastQC (version 0.11.5)