FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446150
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27500.040.5568661
GTGGTAT4550.035.6681821
GGTATCA12000.035.105341
ATCAACG31700.034.834933
TCAACGC31850.034.6708684
CAACGCA32550.034.031285
TATCAAC32900.033.774132
AACGCAG34100.032.6867946
TGGTATC5450.029.7594932
AGAGTAC37800.028.94054211
CGCAGAG38600.028.6983538
ACGCAGA39200.028.4341757
CAGAGTA38800.028.19465410
ATTAGAC1208.501411E-825.8812623
TCGGTTT1551.4354591E-424.37112670
GCAGAGT47250.023.7367239
TGTTAGA4850.023.36613370
AGTACGG39100.023.30056213
GTATAGG1202.4401688E-623.0198941
AGACGAA2202.5567278E-622.89408970
Produced by FastQC (version 0.11.5)