FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446150
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29200.037.7674061
GGTATCA13750.037.5957341
TGGTATC5950.034.166062
GTGGTAT5850.033.5790941
TCAACGC35300.031.039334
CAACGCA35300.030.9417215
TATCAAC35450.030.907992
ATCAACG35650.030.7345943
AACGCAG37050.029.7612846
AGAGTAC39700.027.35396411
CGCAGAG41600.026.4351968
CAGAGTA41600.026.27036710
ACGCAGA42450.025.9843837
CGAACTA2800.023.40000729
AGTACGG39950.023.21323813
GAGTACG41350.022.34392712
GCAGAGT50200.022.0445639
CGTACCG1103.2862306E-521.9384312
TTTAGCG953.0770854E-421.77826
TACGGGG23450.021.3154915
Produced by FastQC (version 0.11.5)