FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889235
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15020.16890923096819177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTA305.8832E-446.6592954
GGTATCA11250.041.812371
GTATCAA27850.041.7937361
TATCAAC30750.037.3887562
CAACGCA32650.035.212995
TCAACGC33000.034.8395234
ATCAACG33450.034.473433
AACGCAG33600.034.219316
ACGCAGA35450.032.5395057
CGCAGAG35800.032.319098
AGAGTAC35500.032.20535711
CAGAGTA36150.031.6262910
GCAGAGT37450.030.7118029
GTCGTAT450.004731820430.5199761
AGTACGG30050.030.50556413
GTGGTAT3750.030.2147771
GAGTACG30550.030.0062912
CGACTAG604.987931E-429.16205854
GTACGGG33250.027.25992214
TACGGGG15450.026.88714815
Produced by FastQC (version 0.11.5)