FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586565
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAATTCTTATTGGTGGAGCTGTATAAAACACT34330.2163794108656122No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT31610.1992354552129916No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26470.1668384213694365No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA25080.15807735579695759No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24370.1536022791376338No Hit
GTTTTATACAGCTCCACCAATAAGAATTATTTAGCTTGGTACCAGCAGAA24100.1519004894221163No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24090.15183746017339347No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24010.1513332261836105No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22870.14414789182920335No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21780.13727770371841053No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21770.13721467446968766No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21350.13456744602332713No Hit
GTAATAAATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAATGAATTCTTGGCGTTGTCTCTGGA19900.12542820495851098No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19470.12271794726342761No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17940.11307447220882852No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17770.11200297498053972No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17730.11175085798564824No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17680.1114357117420339No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17230.10859939554950473No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17220.10853636630078188No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCACGTCATCCAATAAGTAC16610.10469158212868682No Hit
GTCCAGAGACAACGCCAAGAATTCATTGTCTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCATTTATTA16490.10393523114401237No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16190.1020443536823263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.040.3856121
GTGGTAT4200.039.424051
GTATCAA28150.037.2532771
TATCAAC32100.032.6516462
ATCAACG32450.032.405723
TCAACGC32750.032.214154
CAACGCA32950.032.0186165
CGATTGT1401.7922503E-631.90642570
TGGTATC5200.031.8254412
AACGCAG33950.030.8723966
AGAGTAC35800.029.0853411
CAGAGTA36450.028.84953910
CGCAGAG36900.028.4042768
ATATCCG500.0077603927.5820475
GCAGAGT38500.027.223849
ACGCAGA40050.026.0841467
AGTACGG35500.024.76635213
CTGTATA10750.024.4149860
TCGATTG1201.559177E-524.15941869
GAGTACG35700.024.1447112
Produced by FastQC (version 0.11.5)