FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997675
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.040.328851
GTATCAA29150.039.894941
TCGTATG1650.035.6870540
TATCAAC33000.035.2246322
TCAACGC34500.033.792814
ATCAACG34600.033.595753
CAACGCA34850.033.3547445
AACGCAG35400.033.0308236
CTTATAC2850.031.3883271
CGTATGC2000.031.20992341
GTGGTAT4650.031.0767781
ACGCAGA37900.030.850467
CGCAGAG38850.030.0960718
AGAGTAC39750.029.50412211
AATCTCG1900.029.08906736
CAGAGTA41600.028.19204110
TATGCCG2350.028.08696443
GCAGAGT42200.027.9514059
CTCGTAT1653.6379788E-1227.27082339
TGGTATC5050.027.2403262
Produced by FastQC (version 0.11.5)