FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997675
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21350.21399754429047535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.041.1060561
GGTATCA14000.036.8122181
TATCAAC37500.036.2766652
ATCAACG38400.035.158053
TCAACGC38450.035.0229874
TCGACTA400.002431341734.96447454
AACGCAG39950.034.0553556
TGGTATC5550.034.043422
TTTACGC552.0379853E-433.97102468
CAACGCA39750.033.9645
GTGGTAT5850.033.4770931
ACGCAGA41700.032.634357
CGCAGAG43300.031.3538138
AGAGTAC43650.031.1827111
CAGAGTA44250.030.68221910
GCAGAGT46250.029.5040579
CGACTAG500.00722226627.99013955
GCGTTAT500.00783001327.53061726
CGAATTA756.323037E-527.49610515
AGTACGG36750.026.00101913
Produced by FastQC (version 0.11.5)