FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511463
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCCCCAACAATAATAACTACTTAGCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACT46770.9144356483264674No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33190.6489227959793769No Hit
GGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAA28850.5640681730643272No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24250.4741300934769475No Hit
ATATTATAGTTTTCCCTGGGGGTTCGGCCAGGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23840.4661138733398115No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19510.3814547679890823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18820.3679640560509753No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18480.36131645886408204No Hit
GTATAAAAGAGTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGTCACAGCCAGGGAGTCTGGAGA18330.35838369539927617No Hit
GTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGA18270.3572105900133538No Hit
GTCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTATTATTGTTGGGGCTG17170.33570365793811086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16940.3312067539587419No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16100.31478327855582905No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15180.2967956626383531No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15140.29601359238107156No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15000.2932763464805861No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14730.2879973722439355No Hit
CTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC14550.2844780560861685No Hit
GTTTATTACTGTCAACAATATTATAGTTTTCCCTGGGGGTTCGGCCAGGGGACCCAGGTGGAGATCAAACGAA13700.26785906311893526No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13630.26649044016869256No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13620.26629492260437215No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCCCCTGGCCGAACCCC13320.2604293956747604No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13220.25847422003155657No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12980.25378179848786714No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12850.2512400701517021No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12810.2504579998944205No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA12720.24869834181553702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12600.2463521310436923No Hit
GTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGACTCTTTTATA11880.23227486641262418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11840.23149279615534263No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11810.23090624346238145No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTT10620.20763965330825493No Hit
GTCAGGGACCCCGGATTCTCGGGTAGACGCCCAGTAAAAGAGTAGTTTTGGAGGCTGTCCTGGCCTCTGCTGGTA10090.19727722239927425No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9970.19493101162742954No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9660.18886996713349744No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9580.1873058266189343No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9300.18183133481796337No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9300.18183133481796337No Hit
CTGCTGGTACCAAGCTAAGTAGTTATTATTGTTGGGGCTGTATAAAAGAG9300.18183133481796337No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTTCCCTGGGGGTT9270.1812447821250022No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9190.17968064161043906No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9180.1794851240461187No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9100.17792098353155555No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9020.17635684301699242No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8810.17225097416626423No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT8680.16970924583009914No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTTCCCT8580.16775407018689525No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8360.16345268377184666No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8350.16325716620752626No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8260.16149750812864272No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8230.16091095543568157No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8000.15641405145631257No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7910.15465439337742906No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG7820.15289473529854555No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCC7800.15250370016990478No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7740.15133059478398242No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7580.14820231375485615No Hit
CTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCT7560.14781127862621538No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7550.147615761061895No Hit
CTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA7380.14429196246844836No Hit
CTACTTAGCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATC7360.14390092733980756No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA7320.143118857082526No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7290.14253230438956485No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTG7230.1413591990036425No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7190.1405771287463609No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG7070.13823091797451623No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7020.13725333015291427No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6960.13608022476699194No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC6950.13588470720267157No Hit
CTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGACTCTTTTATACAGCCCCAA6910.13510263694539No Hit
GTAAAAGAGTAGTTTTGGAGGCTGTCCTGGCCTCTGCTGGTACCAAGCTA6900.1349071193810696No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGG6850.13392953155946763No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6820.13334297886650648No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC6750.13197435591626375No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6740.13177883835194334No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6710.13119228565898217No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT6640.12982366270873943No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT6570.1284550397584967No Hit
GCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGACCGATT6470.1264998641152928No Hit
CCCAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT6460.1263043465509724No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6400.12513124116505006No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6370.12454468847208888No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6370.12454468847208888No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6340.1239581357791277No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6310.12337158308616655No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT6300.12317606552184615No Hit
CCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT6240.12200296013592381No Hit
CAATAATAACTACTTAGCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTAC6160.12043881962136069No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC6140.12004778449271991No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC6110.11946123179975873No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6050.11828812641383639No Hit
GTTTTGGAGGCTGTCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTATTATTGTTGGGGCTGTATAAAAGAGT6050.11828812641383639No Hit
GCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTC5980.11691950346359366No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5970.11672398589927327No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC5950.11633295077063248No Hit
GCCCCAACAATAATAACTACTTAGCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGG5790.11320466974150624No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5700.1114450116626227No Hit
GGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGA5630.11007638871237999No Hit
GGTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAA5600.10948983601941881No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5440.10636155499029254No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5430.10616603742597217No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG5420.10597051986165178No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG5150.10069154562500124No Hit
GTATCAACGCAGAGTACGGGGGAGCTACAACAGGCAGGCAGGGGCAGCAA5140.10049602806068082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTGC200.00726952451.9062241
CGAGCCC1600.049.35442415
CCCACGA1650.047.868219
TATACAC1800.047.6854363
CGCTCAT1750.047.14126228
ATCTCCG1800.045.7780210
CCGAGCC1800.045.7780214
GCCCACG1750.045.12404618
ACGCTCA1850.044.58872627
TCTCCGA1850.044.54077511
GGTATCA6150.044.1204641
AATCGCG405.0456845E-543.05579436
TACACAT2000.042.916895
CACGAGA1900.041.57787721
CGTATGC758.440111E-1041.5249841
AATCTCG1000.041.33356536
TCGTATG602.8625982E-740.327840
CTCGTAT602.8781142E-740.30003439
GTATCAA14750.040.052031
CTTATAC2100.039.253651
Produced by FastQC (version 0.11.5)