FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511463
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCCCCAACAATAATAACTACTTAGCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACT36520.7140301448980669No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34080.6663238592038916No Hit
GGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAA24860.4860566649004913No Hit
ATATTATAGTTTTCCCTGGGGGTTCGGCCAGGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCAT21280.4160613768737915No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20480.40041997172816024No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20210.3951409974915097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20190.39474996236286886No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20110.3931858218483057No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18630.36424922232888796No Hit
GTCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTATTATTGTTGGGGCTG17740.34684815910437317No Hit
GTATAAAAGAGTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGTCACAGCCAGGGAGTCTGGAGA17520.34254677268932454No Hit
CTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC17460.3413736673034022No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15510.303247742260926No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15160.2964046275097123No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14880.2909301357087414No Hit
GTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGA14440.2823273628786442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14400.2815452926213627No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCCCCTGGCCGAACCCC14140.27646183594903245No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13930.2723559670983043No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13750.2688366509405372No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13580.2655128523470906No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13310.26023387811044No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13220.25847422003155657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13040.2549549038737895No Hit
GTTTATTACTGTCAACAATATTATAGTTTTCCCTGGGGGTTCGGCCAGGGGACCCAGGTGGAGATCAAACGAA12560.24557006078641075No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12490.24420143783616802No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12320.24087763924272138No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11920.23305693666990576No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11840.23149279615534263No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC11720.2291465853834979No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10920.2135051802378667No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10820.21155000459466278No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC10680.2088127586941773No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10640.20803068843689576No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCTCGGGT10620.20763965330825493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10610.20744413574393458No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10200.1994279156067985No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10070.19688618727063345No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9970.19493101162742954No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTT9740.19043410764806057No Hit
CTGCTGGTACCAAGCTAAGTAGTTATTATTGTTGGGGCTGTATAAAAGAG9690.1894565198264586No Hit
GTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGACTCTTTTATA9650.18867444956917706No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTTCCCT8900.17401063224514773No Hit
GTCAGGGACCCCGGATTCTCGGGTAGACGCCCAGTAAAAGAGTAGTTTTGGAGGCTGTCCTGGCCTCTGCTGGTA8800.17205545660194382No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8740.1708823512160215No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8690.16990476339441954No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTTTTCCCTGGGGGTT8580.16775407018689525No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8540.16697199992961367No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA8300.1622795783859243No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG8270.16169302569296315No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8130.15895577979247766No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7980.1560230163276718No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA7910.15465439337742906No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7860.1536768055558271No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7850.15348128799150673No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7630.14917990157645813No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7610.14878886644781733No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG7420.1450740327257299No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7340.1435098922111668No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7330.1433143746468464No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7040.13764436528155508No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7030.1374488477172347No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT6980.13647125989563272No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6970.13627574233131234No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6950.13588470720267157No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6670.1304102154017006No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6580.1286505573228171No Hit
CTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCT6560.12825952219417633No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT6480.1266953816796132No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6460.1263043465509724No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6310.12337158308616655No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6280.12278503039320537No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCTCGGGTAGA6280.12278503039320537No Hit
CCCAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT6220.12161192500728304No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT6090.11907019667111796No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6080.11887467910679755No Hit
CTTTTACTGGGCGTCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA6070.11867916154247717No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA6060.11848364397815678No Hit
CTACTTAGCTTGGTACCAGCAGAGGCCAGGACAGCCTCCAAAACTACTCTTTTACTGGGCGTCTACCCGAGAATC6030.1178970912851956No Hit
GTAAAAGAGTAGTTTTGGAGGCTGTCCTGGCCTCTGCTGGTACCAAGCTA6020.11770157372087521No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGG5990.11711502102791403No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5980.11691950346359366No Hit
GTTTTGGAGGCTGTCCTGGCCTCTGCTGGTACCAAGCTAAGTAGTTATTATTGTTGGGGCTGTATAAAAGAGT5970.11672398589927327No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5870.11476881025606935No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5830.11398673999878778No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG5800.11340018730582663No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5660.11066294140534114No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5590.10929431845509842No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCC5570.10890328332645763No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT5540.10831673063349646No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCCCCTGGCCGAACCCCCAGGGAAAA5530.10812121306917606No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC5520.10792569550485567No Hit
CTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGACTCTTTTATACAGCCCCAA5490.10733914281189452No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTG5470.10694810768325372No Hit
GGTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAA5450.10655707255461296No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5320.10401534421844788No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC5310.10381982665412746No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5290.1034287915254867No Hit
CCCTGGCTGTGACTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT5230.10225568613956434No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC5120.10010499293204006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)