FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320497
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC80820.3482874573852067No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA71290.3072186691040756No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT70260.30277996480926284No Hit
GCCTTGGGCTGACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGGCTGTCATCCCATG68790.2964451149904525No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC61240.2639089815673108No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC57570.24809340412851213No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG57100.24606797595515098No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT51070.22008216343309214No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC50040.21564345913827943No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC46780.20159474457411494No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC46280.19944003375139036No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT45990.19819030147421005No Hit
GTAATAATCACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGC44420.19142450949085477No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG40650.17517798988751115No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGCGACCTTGGTC39520.17030834342815354No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC39320.1694464590990637No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38100.16418896469161565No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC37980.16367183409416172No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG37900.1633270803625258No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37260.1605690505094383No Hit
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGG36090.15552702718426267No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35540.1531568452792656No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT35400.15255352624890273No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34550.14889051785027088No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT34030.14664961859463727No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC34030.14664961859463727No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG33260.14333136392764137No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32580.14040095720873588No Hit
CCCCTGAGGGCCGCTGACTATTAGTAAAGATGAGGAGTTTGGGGGCCGTT32230.13889265963282865No Hit
GTCTGAGGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGAC31930.13759983313919388No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC30700.13229924451529132No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG30220.1302307221254757No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29480.12704175010784327No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGT29010.12501632193448214No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA28940.1247146624193007No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28940.1247146624193007No Hit
CTGATGGCCAGGGAGCCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC28460.12264614002948505No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27920.12031905234094248No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG27750.1195864506612161No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27070.11665604394231063No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC27050.11656985550940165No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTCGCTTTGCC26970.11622510177776571No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT26880.11583725382967529No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG25820.1112692668854991No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA25650.11053666520577272No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCA25030.10786482378559421No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24670.10631343199323248No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG24640.10618414934386901No Hit
GACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGG24200.10428800381987134No Hit
ATCCCATGCTGAACAGTAATAATCACCCTCATCCTCAGACTGGAGCCCAC24190.10424490960341684No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24110.1039001558717809No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT24090.10381396743887193No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC23930.10312445997560005No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23840.10273661202750961No Hit
GTCTGGCACCTCAGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG23650.10191782191487427No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA23410.10088356071996644No Hit
GCATGGGATGACAGCCTGAATGGTCACGTCTTCGGAACTGGGACCAAGGT23410.10088356071996644No Hit
GTAGTAGCATCAGTTGCTACAAATATTCTGACTACTGGGGCCAGGGGACCCTGGTCACCGTCTCCTCAGCCTCCA23350.1006249954212395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.042.3113821
GTATCAA35650.040.672611
TGGTATC5950.040.5911372
GTGGTAT6000.039.12641
TATCAAC40150.035.748512
ATCAACG42650.033.976643
TCAACGC43050.033.58084
CAACGCA43450.033.430475
AACGCAG43900.033.0877886
CGCAGAG48050.030.1608438
AGAGTAC48300.030.00602311
CAGAGTA49250.029.42722710
GCAGAGT51400.028.1951089
CATACCG500.007733817727.6019735
AGTACGG43750.025.63371813
GAGTACG44500.025.20168912
GTGTCCA22450.025.17523870
AGCGTCA2200.023.5244083
CGCTAAT1052.2652832E-523.02198432
GTACGGG49500.022.3772214
Produced by FastQC (version 0.11.5)