FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043763
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG453.150737E-668.7835270
GTATCAA28400.043.839521
GGTATCA11050.041.8234141
TATCGAT503.716099E-641.60185242
TATGCGA650.001595366338.0954970
ATCAACG34750.035.6044583
TCAACGC34950.035.4993254
CAACGCA35150.035.2973375
TATCAAC35650.034.6089362
AACGCAG37200.033.352196
ACGCAGA38550.032.182677
TCGATTA652.2335826E-532.0261244
CGCAGAG38800.031.9753078
AGAGTAC39600.031.41636711
CAGAGTA40100.031.19652410
TCGGTAC450.00464621430.6346023
CGATTAC703.6944388E-529.7514545
GCAGAGT42800.029.3895579
GTAAGCG605.21347E-428.94317646
AGTACGG36300.026.58247213
Produced by FastQC (version 0.11.5)