FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269315
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6700.039.0440331
GGTATCA3200.037.647311
GTGGTAT1151.8189894E-1235.916941
TACATCG400.00253511234.65862341
TCAACGC8350.031.3112954
CAACGCA8500.030.7587435
GATTCGC450.004647746730.62435530
TCGTAGT450.00466892930.59590726
GGTAGTC450.00467317530.59022324
ATCAACG8600.030.4010853
AACGCAG8750.029.8799216
CAACGTT550.005241433629.87865369
TATCAAC8850.029.5422952
ATTAACC703.916058E-529.4867673
ACGCAGA8950.029.2122127
CCTACGC605.4849515E-428.68366626
GTCAATT605.490919E-428.67833713
GCCTATA500.00783340927.520982
CAGAGTA9500.027.16895110
CGCAGAG9600.026.8809498
Produced by FastQC (version 0.11.5)