FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269315
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3060.11362159552939866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT200.00674544452.89057564
TTCGATC200.00743093151.6120821
GATACCG200.007469254551.5449875
TCGATCC503.897745E-641.2973422
GTATCAA7900.040.90321
CCCATAC350.00137375739.265083
CAACGCA9200.034.3633235
TCAACGC9200.034.356944
TATCAAC9350.033.8057632
ATCAACG9350.033.8057633
ACGCAGA9500.033.3090937
AACGCAG9600.032.9437566
GGTATCA3650.032.0215641
AGAGTAC10250.030.87184311
CGCAGAG10400.030.4265778
TACGGGT1600.030.09585415
CAGAGTA10500.029.80922710
TACGGGG4250.029.13480815
GCAGAGT11300.028.3076029
GAGTACG9500.028.2403212
Produced by FastQC (version 0.11.5)