FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960191
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91640.46750546247789115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67950.040.4267271
GGTATCA27850.038.6494751
TATCAAC76800.035.628272
TCAACGC78750.034.659834
ATCAACG79400.034.376093
CAACGCA81000.033.6970565
AACGCAG84000.032.537326
ACGCAGA90350.030.2237427
CGCAGAG91300.029.803748
AGAGTAC91500.029.7772411
AGTACGG60000.029.49970413
CAGAGTA92950.029.31197410
GAGTACG61250.028.95313612
GCAGAGT96550.028.2190389
GTACGGG65400.027.01131414
TACGGGA23050.026.97188815
GTGGTAT12200.026.7190461
TGGTATC12050.024.7922822
TACGGGG25900.024.52861615
ACGGGAT6000.023.21118416
Produced by FastQC (version 0.11.5)