FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868134
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20900.24074624424340021No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACTTCATCACTA19400.223467805661338No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACTTCATCACTACTACTATCCCACAC19360.2230070472991497No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18370.2116032778349886No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16990.1957071143394914No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG16980.1955919247489443No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT15480.17831348616688208No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCC15440.17785272780469374No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14730.16967426687585097No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC13450.15492999928582454No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12920.1488249509868292No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12910.14870976139628214No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12770.14709710712862298No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12530.14433255695549305No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACACTTTTACTT12460.1435262298216635No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCCAC12340.1421439547350985No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12320.14191357555400433No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11830.13626928561719734No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGT11670.13442625216844403No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11020.12693892878288374No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10890.12544146410577167No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10640.12256172434209467No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10540.12140982843662385No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10470.12060350130279426No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10310.11876046785404097No Hit
GGATGGCCGTGTTCCCATCCTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA10000.11518959054708144No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG9890.11392250505106354No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCCACCCTGC9770.11254022996449857No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9610.11069719651574526No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9500.10943011101972736No Hit
GGATAGTAGTAGTGATGAAGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC9490.10931492142918028No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGGATGGGAACACGG9380.10804783593316239No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9310.10724150879933284No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9190.10585923371276786No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT9180.10574404412222076No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC8930.10286430435854373No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8890.1024035459963554No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8730.1005605125476021No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8730.1005605125476021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA150.002373551568.8661921
AAACGTT300.003203804163.8426370
CTTATAC2800.045.52711
TATACAC3000.043.612743
GTATCAA16000.041.343531
TTATACA3200.040.8869442
GGTATCA7050.040.072921
TATAGTG350.001360942139.349845
TATCAAC18000.036.91782
ATCAACG18800.035.1636853
TCAACGC19000.034.7935454
CAACGCA19250.034.1628155
AACGCAG19550.033.6385776
TGGTATC2100.032.7915342
CACGAGA3850.032.19717821
AGAGTAC20600.032.0929811
CGAGACT4100.031.91727623
TACACAT4500.031.3705655
ACGAGAC4100.031.07734922
CAGAGTA21300.031.0382810
Produced by FastQC (version 0.11.5)