FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425119
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT6060.1425483217640237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT253.0368756E-669.2056439
TCGCGTA253.0561914E-669.1318138
TCGTATG1500.050.79903440
GCGTATG352.2183067E-549.47957640
TTATACA2500.049.3885082
CGTATGC1850.046.83838741
TATACAC2700.044.459823
ACGAGAC2750.043.6771522
CTTATAC2800.042.8971981
TGCGTGA503.4174973E-642.10294755
TCTGTCG759.2040864E-1041.1570938
ATCTCCG2850.040.91640510
GTATCAA10700.040.7322921
CTCGTAT1900.040.0664339
CGAGACA3050.039.39030523
CGAGCCC3150.039.1972315
TCTCCGA3000.038.8705911
AGGGGGG2250.038.4975170
TACACAT3300.038.454865
GGTATCA4900.037.819571
Produced by FastQC (version 0.11.5)