FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309379
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40000.038.2658841
GGTATCA19300.036.9744571
TATCAAC44300.034.4527052
TCAACGC45200.034.071594
CAACGCA45650.033.5847855
ATCAACG46650.032.938713
AACGCAG47150.032.4432686
ACGCAGA49700.030.8479967
ATCGCGT450.00456432430.74673737
CGCAGAG50750.030.1453298
AGAGTAC50950.029.9605111
CAGAGTA52100.029.36533410
TCGTATG953.0902447E-729.17322540
GCAGAGT54600.028.0828049
ACGGGTA1404.7475623E-1027.0720516
AGTACGG43750.026.62124313
GAGTACG44200.026.27225312
GGCGAAT2500.026.22110432
TAAGGCG2550.025.68437429
CGTACGC1109.726991E-725.58507763
Produced by FastQC (version 0.11.5)