FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309379
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15220.11623830838893857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40200.041.3091
GGTATCA17600.038.6790771
TATCAAC47750.034.3388442
CAACGCA48750.033.493435
TCAACGC48750.033.422924
AACGCAG49700.032.7186366
ATCAACG50450.032.364813
ACGCAGA52900.030.751187
CGTCATA450.00460042430.69725835
CGCAGAG53750.030.4661488
AGAGTAC54600.030.05601511
CAGAGTA56500.029.10617410
GCAGAGT58900.027.9201839
GTGGTAT6050.027.8460941
AGTACGG46350.027.09248413
GAGTACG46450.027.03415712
TAACGGC2950.026.94167336
TTAACGG2950.026.92513735
TGGTATC6550.024.6659152
TACGGGG22350.024.31935515
Produced by FastQC (version 0.11.5)