FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938396
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTACACTATCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAAACGAACTGTGGCTGCACCAT54640.5822701716546107No Hit
GTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC54210.577687884432585No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48060.5121505206757062No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44440.47357405615539705No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39120.416881572385219No Hit
ACACTATCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAAACGA38480.410061423961739No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35680.3802232746090137No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35260.3757475522061049No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34940.37233747799436484No Hit
CTCCTGATCTATGCCACATCCACTTTGCAAAGTGGTGTCCCATCAAGGTT34510.3677551907723392No Hit
GATCAGGAGTTTGGGGGCTTTCGCATGTTTCTGCTGATACCAATTTAAAT34330.3658370240282354No Hit
GGATAGTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT31400.33461353202699073No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30570.32576865204029No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30360.3235307908388356No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29490.3142596515756674No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29360.312874308927148No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25580.2725928073009689No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24830.26460044586720316No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24480.2608706771981125No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24270.25863281599665816No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24150.2573540381672556No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23560.2510667138393599No Hit
GTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT22850.24350061168206175No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22340.23806580590710105No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22290.23753298181151666No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGAGCATCGACAA22190.23646733362034789No Hit
GGACACCACTTTGCAAAGTGGATGTGGCATAGATCAGGAGTTTGGGGGCT22160.23614763916299727No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22110.2356148150674129No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21930.23369664832330914No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21900.23337695386595853No Hit
ATCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC21630.23049970374980286No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20310.21643314762637522No Hit
ATAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGAGCATCGACAACTATTTAAATTGGTATCAGCAGAAA20080.21398215678668708No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19860.21163773076611578No Hit
GTGCAGCCACAGTTCGTTTAATATCCAGTCGTGTCCCTTGGCCGAAGGTG19730.2102523881175964No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19260.20524384161910322No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18260.1945873597074156No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18250.19448079488829875No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17450.18595560935894867No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17410.18552935008248117No Hit
GGACACGACTGGATATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17260.18393087779572803No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACACCA17170.18297179442367614No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16590.17679103491489734No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16380.17455317371344295No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16200.17263500696933917No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16130.17188905323552103No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16080.17135622913993664No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15960.17007745131053414No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15830.16869210866201476No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACACTATC15760.16794615492819662No Hit
CCTTCGGCCAAGGGACACGACTGGATATTAAACGAACTGTGGCTGCACCA15620.16645424746056037No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACACCACTTTGCAAAGT15540.16560172890762534No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15400.1641098214399891No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15110.1610194416855997No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG15060.1604866175900153No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14950.15931440457972967No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14720.15686341374004154No Hit
ACTTACTACTGTCAACAGACTTACACTATCCCGATCACCTTCGGCCAAGGGACACGACTGGATATTAAACGAA14450.15398616362388587No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14360.15302708025183398No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14240.15174830242243145No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACACTATCCCGATCACCTT14200.15132204314596398No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14040.14961700604009395No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13860.14769883929599018No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGATAGTGTAAGTCTGTTGACAG13840.14748570965775643No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13680.1457806725518864No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT13660.14556754291365268No Hit
GTCTCCTATAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT13490.1437559409886658No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13490.1437559409886658No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13460.14343624653131512No Hit
GATCGGGATAGTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG13360.14237059834014637No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13290.14162464460632823No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12960.13810800557547134No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12910.13757518147988695No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12780.13618983883136757No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12710.13544388509754945No Hit
GCTCTGACTTGCCCGGCAACTGATGGTGACT12520.1334191535343288No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12330.13139442197110815No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGA12290.13096816269464065No Hit
GTGGATGTGGCATAGATCAGGAGTTTGGGGGCTTTCGCATGTTTCTGCTGATACCAATTTAAATAGTTGTCGATG12220.1302222089608225No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12180.129795949684355No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCA12130.12926312558877062No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12100.12894343113142No Hit
CAGCAGAAACATGCGAAAGCCCCCAAACTCCTGATCTATGCCACATCCAC12080.12873030149318623No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA12020.128090912578485No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACACTATCCCGATCACCTTCGGCCAAGGGA12000.12787778294025123No Hit
ATGCCACATCCACTTTGCAAAGTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA11760.1253202272814462No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11700.12468083836674497No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11570.12329549571822557No Hit
ACCTTGATGGGACACCACTTTGCAAAGTGGATGTGGCATAGATCAGGAGTTTGGGGGCTTTCGCATGTTTCTG11400.12148389379323866No Hit
CTATAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGAGCATCGAC11180.1191394677726674No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG11160.11892633813443364No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11120.11850007885796615No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCAGTTGCCG11110.11839351403884926No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11100.11828694921973239No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11020.11743443066679739No Hit
CGACTGGATATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT10880.11594252319916112No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10750.11455718055064174No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC10730.11434405091240799No Hit
GATCTATGCCACATCCACTTTGCAAAGTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA10690.11391779163594047No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10660.11359809717858985No Hit
TTGCAAAGTGGTGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTG10600.1129587082638886No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10260.10933550441391482No Hit
GGCCGAAGGTGATCGGGATAGTGTAAGTCTGTTGACAGTAGTAAGTTGCA10060.10720420803157729No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10040.10699107839334354No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9990.10645825429775917No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9920.10571230056394103No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT9860.10507291164923976No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9810.10454008755365538No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGA9580.10208909671396724No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9510.1013431429801491No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATATCCAGTCGTGTCC9430.1004906244272141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)