FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035811
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTAACGA78980.3879534986302756No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGCGTGCTTGTATATGAA74320.3650633580425688No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGC69150.3396680733132889No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG62860.30877129556722116No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62140.30523462148500036No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC62080.3049398986448153No Hit
TCTCAAGACTCCGGGCTGAGGACGAGGCTGATTATTCCTGCAGTTCATAT61300.3011085017224094No Hit
GTCTTGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG56550.27777627687442497No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC50860.24982672753020788No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50740.24923728184983773No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG49810.24466907782696923No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT49420.24275337936576624No Hit
GTGTATTACTGTGCGAAATCGGAAATTGCCCTCCGTACTGGGAGCTACTA49320.24226217463212452No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43370.21303549298043878No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41590.20429204872161513No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41040.20159042268658536No Hit
GTTCATATACAAGCACGCGCACGCTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG40270.19780814623754367No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAATGC39800.1954994839894273No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39180.1924540146408483No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC38800.19058743665300953No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC38300.18813141298480066No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA37590.18464385937594405No Hit
GTATATGAACTGCAGGAATAATCAGCCTCGTCCTCAGCCCGGAGTCTTGA37080.18213871523437097No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT36620.1798791734596188No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35200.17290406624190557No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35140.1726093434017205No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT34730.17059540399378922No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG34150.1677464165386669No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33870.16637104328446992No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33640.16524127239709382No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC32760.16091867074104618No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGCGT32000.15718551476536868No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31850.156448707664906No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC31330.15389444304996877No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31040.15246994932240765No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30190.1482947090864525No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29850.1466246129920705No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29770.14623164920515708No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG29260.143726505063584No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGCG29180.14333354127667058No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT29120.1430388184364855No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29040.14264585464957208No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC29000.14244937275611536No Hit
CACCTGCACTGTCGCTGGTGACTCCATCAGCAGTAGTGGTTACTACTGGG29000.14244937275611536No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28600.14048455382154826No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC28370.13935478293417217No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGTGGTGGTCTCCA28010.13758644589306177No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG27590.13552338601176633No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27300.13409889228420516No Hit
AGCCACGAGCGTGCGCGTGCTTGTATATGAACTGCAGGAATAATCAGCCT27300.13409889228420516No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC27290.134049771810841No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGAAA26730.13129902530244703No Hit
CTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC26220.12879388116087395No Hit
CTTGTATATGAACTGCAGGAATAATCAGCCTCGTCCTCAGCCCGGAGTCTTGAGATGGTCAGGGAGGCCGTGTTG26150.12845003784732473No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26020.12781147169359042No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25160.12358711098427112No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24790.12176965346979654No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC24420.11995219595532199No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC24370.1197065935885011No Hit
CCACCACACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAT24300.11936275027495186No Hit
CTACTGGGGCTGGCTCCGCCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGAGTATCTTTTACGATGGGAGCCC24000.11788913607402651No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23800.11690672660674295No Hit
CAGTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGA23340.11464718483199078No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTC23310.11449982341189827No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23100.11346829347125052No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23020.1130753296843371No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22530.11066842648949238No Hit
CTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGG22380.10993161938902972No Hit
ATATACAAGCACGCGCACGCTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGC22310.1095877760754805No Hit
GTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTC22230.10919481228856705No Hit
GATTATTCCTGCAGTTCATATACAAGCACGCGCACGCTCGTGGCTTTTGG22160.10885096897501782No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC21890.10752471619418502No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC21640.10629670436008058No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21310.10467572873906271No Hit
CTCTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC21210.10418452400542094No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21090.1035950783250508No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21030.10330035548486574No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG21000.1031529940647732No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA20920.10276003027785978No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20740.10187586175730459No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA20710.10172850033721205No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT20440.10040224755637926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4050.045.9079251
GGTATCA14000.043.530551
GTATCAA35350.041.8820531
TATACAC1750.041.2927783
TGGTATC4550.040.8390122
CTCGTAT856.548362E-1140.6528639
TCGTATG1150.039.08619740
CGTATGC1200.037.4816541
CTTATAC1850.037.2226371
TATCAAC40200.036.8930052
ATCAACG42300.035.2241363
TCAACGC42800.034.651844
TCTCGTA1003.765308E-1034.5361938
TACACAT1900.034.4106455
CAACGCA43250.034.3708655
AACGCAG43700.034.1744166
TCGACGT2000.032.70537629
CTCGACG2000.032.70296528
AGCGTCA1600.032.259983
CGCAGAG46650.032.085528
Produced by FastQC (version 0.11.5)