FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713050
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGATCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT18800.26365612509641684No Hit
CTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGCTTATGGTGAGAGTGAAATCTGTC15250.2138699950915083No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14360.20138840193534815No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12910.18105322207418834No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12170.17067526821401025No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11230.1574924619591894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10790.1513217866909754No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9730.1364560689993689No Hit
GGTTAGGAGGCTGTCCGGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGTGGGAGCTGGATAAAACACT9360.13126709206927986No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8710.12215132178669097No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8710.12215132178669097No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8220.11527943341981628No Hit
GTTTTATCCAGCTCCCACAATAAGAACTACTTAGCTTGGTACCAACAGAA7880.11051118434892364No Hit
CACCATAAGCAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGC7880.11051118434892364No Hit
CTCCTAACCTACTCATTTACTGGTCATCTACCCGGGAATCCGGGGTCCCT7670.10756608933454877No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7650.1072856040950845No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7190.1008344435874062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2900.047.356341
CGCTAGA306.4890634E-445.739272
ACGTTAG703.8395825E-545.09167570
GGTATCA10850.042.0860331
GTATCAA28300.041.9764561
CGATTGT1302.4876499E-838.84820670
TGGTATC3850.036.5320172
TATCAAC32500.036.204392
ATCAACG33550.035.071323
TCAACGC33700.034.9152154
CAACGCA34100.034.5056535
AACGCAG34700.033.909016
AGAGTAC36800.031.97174511
CGCAGAG37350.031.5009428
CAGAGTA37750.031.1671610
ACGCAGA38000.031.142657
GCAGAGT38150.031.1101139
CGGGATA1700.028.25072517
ACGGGAT5300.027.82996216
TCGTATG906.7322E-626.91909440
Produced by FastQC (version 0.11.5)