FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207194
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34900.041.4774741
GGTATCA14900.039.0920871
TCAACGC40500.035.2934074
ATCAACG40600.035.206483
CAACGCA41500.034.6089555
TATCAAC41900.034.360762
AACGCAG42850.033.598976
GTGGTAT5200.033.1401981
ACGCAGA45050.031.958187
AGAGTAC46550.031.22563411
CGACTAA450.00428217631.15012660
CGCAGAG46600.030.896478
CAGAGTA47300.030.65769210
GCAGAGT50650.028.5619859
GAGTACG41000.028.05957612
AGTACGG41000.027.89155413
TCGTATG951.0190894E-525.52173640
ACGGGTA2600.025.170916
GTACGGG45900.024.83898414
TACGGGG18950.024.35544815
Produced by FastQC (version 0.11.5)