FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207194
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38050.039.849641
GTGGTAT7650.036.85471
GGTATCA16550.036.1486171
TCAACGC44950.033.496154
ATCAACG45300.033.4650043
TATCAAC45850.033.1385422
CAACGCA45550.033.0549285
AACGCAG45900.032.9594886
TGGTATC8300.032.718962
ACGCAGA48100.031.4715427
CGCAGAG49400.030.9244828
AGAGTAC49500.030.72426211
CAGAGTA50250.030.40264310
GCAGAGT54050.028.3913219
AATTACG500.00750869327.76848444
AGTACGG45800.026.4448813
GAGTACG46450.026.148912
GTACGGG51250.023.42933514
ATTACGT750.001918160423.15202145
TACGGGG19650.023.1121215
Produced by FastQC (version 0.11.5)