FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449047
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51720.21118418715524856No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACAGCCA48710.1988936921177911No Hit
GACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACAGCCAAAGGTCGCCA48280.1971379071124401No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG45290.18492907649383616No Hit
AGCCAGGACAGGCCCCTATCCTTGTCTTCTATGGTAAAAACAATCGGCCC44660.18235664729994974No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40870.16688123992720433No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC37390.15267163104668877No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36720.14993587301509526No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36470.14891506777942604No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT35630.14548516218757745No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35490.14491351125560267No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35010.14295356520311778No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34260.13989114949611012No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31400.12821313760005423No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTC30790.12572237282502133No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTTAGGGAGA30720.12543654735903395No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30490.12449740654221828No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG29480.1203733533901146No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG28760.11743343431138724No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAA28410.11600430698145033No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26060.10640873776615964No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA25890.10571459020590457No Hit
CCCCTATCCTTGTCTTCTATGGTAAAAACAATCGGCCCTCAGGGATCCCA25650.10473461717966212No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG25190.10285633554603076No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25160.10273383891775045No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24710.10089638949354585No Hit
GTATATTACTGTGCGAGAGAGGCTTATGATAGAAGTGGCCCTTGGGGCCA24500.1000389130955837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.046.440731
GTATCAA55750.042.54371
TATCAAC61700.038.1874242
ATCAACG63100.037.3946723
TCAACGC63350.037.1928024
CAACGCA64250.036.7788855
AACGCAG65700.036.071886
TCGTATG2250.033.84293740
CGCAGAG72500.032.5930378
TCTCGTA2250.032.25227438
AGAGTAC73650.032.03937511
CAGAGTA75600.031.30396310
ACGCAGA77050.030.7123497
TATACAC7200.030.5747173
ACGAGAC5750.030.51323522
CTTATAC6450.029.353771
GTGGTAT5650.028.0265941
GACCGGA1355.9112892E-527.77035170
TTATACA7400.027.4242822
AGTACGG55350.027.22006613
Produced by FastQC (version 0.11.5)