FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449047
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57070.23302941919856987No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48390.19758706141613452No Hit
GACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACAGCCAAAGGTCGCCA43600.17802843310071223No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTCCGCCGAACAGCCA43220.17647680914249503No Hit
AGCCAGGACAGGCCCCTATCCTTGTCTTCTATGGTAAAAACAATCGGCCC41020.16749372306860585No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39640.16185887816771174No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38780.15834730815700965No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37910.15479490593688075No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC37160.1517324902298731No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35950.14679179288923405No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35620.14544432997815068No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33360.13621625064770093No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32870.13421547238578924No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32440.13245968738043817No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32160.13131638551648867No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTTAGGGAGA31580.12894811736973605No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29450.1202508567618343No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG28530.11649429349457156No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTC28200.11514683058348818No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA27110.11069611975597039No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26940.11000197219571532No Hit
CCCCTATCCTTGTCTTCTATGGTAAAAACAATCGGCCCTCAGGGATCCCA26380.10771536846781626No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26150.10677622765100057No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAA25520.10420379845711414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.045.431551
GTATCAA54400.039.9142841
GTGGTAT6550.037.766031
TATCAAC58950.036.7124752
ATCAACG61200.035.4188843
TCAACGC61350.035.22034
CAACGCA62650.034.54435
AACGCAG63850.034.0068446
TGGTATC7300.033.411232
AGAGTAC69650.030.99665311
CGCAGAG70150.030.871218
CAGAGTA72550.029.80502710
ACGCAGA74600.029.1170967
CACTGAT17200.028.9114370
TTACGAA900.00680416628.33484870
AGTACGG56900.025.79834613
GAGTACG57850.025.49354212
TTCGGGG14450.025.14840370
GCAGAGT89000.024.489259
GGCGCTA902.0621571E-423.10585642
Produced by FastQC (version 0.11.5)