FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585063
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21650.041.4850851
GGTATCA8500.039.674961
TATCAAC25450.035.672082
CCGTCTA400.00241477635.0107955
CAACGCA26050.034.4544335
ATCAACG26350.034.453683
TCAACGC26700.033.8732454
AACGCAG27000.033.2421466
TACGGGT3850.033.0514415
AAGTCCG750.00336818632.7087570
TAACCGT652.122871E-532.2615153
ACGCAGA28550.031.4374077
AGAGTAC29000.031.0708211
TAAGCGG450.00464578630.63294231
GTCACGA450.00467897230.5884041
CGCAGAG29800.030.1187258
CAGAGTA30050.029.87070710
AGTACGG23550.029.06107113
GAGTACG24100.028.68325812
GCAGAGT31350.028.62969
Produced by FastQC (version 0.11.5)