FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741228
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11150.15042604974447807No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11050.14907693719071594No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10610.14314084195416255No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10310.13909350429287615No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9510.12830060386277906No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG9190.12398344369074024No Hit
GTATGATAATCTCGTTCGCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT7990.10779409304559462No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7650.10320711036280335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG451.8158207E-646.1626240
CTCGTAT451.8226146E-646.13760839
GTATCAA18450.042.600081
TATCAAC20950.037.5013542
ATCAACG21600.036.213313
GTGGTAT2600.035.7982941
TCAACGC22250.035.0005264
CAACGCA22350.034.843925
AACGCAG22650.034.3824126
AGAGTAC24700.031.38930311
CAGAGTA25050.030.81317310
CGCAGAG25500.030.4045438
ACGCAGA26050.029.894887
GGTATCA9900.029.5975441
GCAGAGT26600.029.2767529
GTATATC605.4586946E-428.7153994
TAGCTAG500.00743411427.82392549
CTTATAC906.944778E-626.8118921
CGTATGC809.2835166E-525.98584741
AGTACGG24750.025.8960713
Produced by FastQC (version 0.11.5)