FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365432
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39200.043.1972051
GGTATCA14250.038.160921
TATCAAC45900.036.6466452
ATCAACG45900.036.6466453
TCAACGC45750.036.6164174
CAACGCA46950.035.827075
AACGCAG48650.034.5044446
TCGTATG958.469215E-932.81263440
ACGCAGA51900.032.3437547
CGCAGAG52300.032.1633348
AGAGTAC52550.032.01266511
CAGAGTA53300.031.5610510
GCAGAGT57350.029.451139
AGTACGG39600.028.84221613
TGGTATC6250.028.6194522
GAGTACG40450.028.23613712
GTGGTAT6200.027.7558751
CGTACGT500.00783075327.53080223
CGTATAC907.067474E-626.7542973
GTACGGG44850.025.15921614
Produced by FastQC (version 0.11.5)