FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4663
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT3968.4923868754021No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT1022.187432983058117No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT942.0158696118378727No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT721.5440703409822003Illumina PCR Primer Index 7 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGT290.6219172206733862No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTTCT210.4503538494531417RNA PCR Primer, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGCGTATGCCGT190.4074630066480806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.4074630066480806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.4074630066480806No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC180.3860175852455501No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.3860175852455501No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG180.3860175852455501RNA PCR Primer, Index 41 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.3860175852455501No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTT170.3645721638430195No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.34312674244048896No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGG150.3216813210379584No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG140.30023589963542785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140.30023589963542785No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.30023589963542785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.30023589963542785No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGGCGT130.27879047823289727No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.27879047823289727No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCA130.27879047823289727No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT120.25734505683036674No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.25734505683036674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.25734505683036674No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGGCGT120.25734505683036674No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.23589963542783618No Hit
GGTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG110.23589963542783618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.23589963542783618No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC110.23589963542783618RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110.23589963542783618No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTTTGCCGT100.2144542140253056No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTGT100.2144542140253056RNA PCR Primer, Index 7 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2144542140253056No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCG100.2144542140253056No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGG100.2144542140253056No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTTTGCCGT100.2144542140253056No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2144542140253056No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC90.19300879262277504No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATACC90.19300879262277504No Hit
TCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC90.19300879262277504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19300879262277504No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19300879262277504No Hit
CGCCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC90.19300879262277504No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19300879262277504No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGGATGCCGT90.19300879262277504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90.19300879262277504No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATACCAC80.17156337122024448No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGACAAAAA80.17156337122024448No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTT80.17156337122024448No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80.17156337122024448No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17156337122024448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17156337122024448No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGT80.17156337122024448No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGGCTT80.17156337122024448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17156337122024448No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15011794981771392No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGGCGTCTT70.15011794981771392No Hit
CCCGTACTCTGCGTTGATAC70.15011794981771392No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15011794981771392No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGG70.15011794981771392No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGAGAAAAA70.15011794981771392No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTC70.15011794981771392No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGCGTATGCCGG60.12867252841518337No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12867252841518337No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12867252841518337No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12867252841518337No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCT60.12867252841518337No Hit
GTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCGACGTCATCT60.12867252841518337No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGC60.12867252841518337No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12867252841518337No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTTGT60.12867252841518337No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12867252841518337No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGT60.12867252841518337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12867252841518337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12867252841518337No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12867252841518337No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCCT50.1072271070126528No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1072271070126528No Hit
GGTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGACAAAA50.1072271070126528No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1072271070126528No Hit
GGTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCG50.1072271070126528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1072271070126528No Hit
GAGAAATGGCGGGACAAAAAAAAAAAAAAAAAAAAAAAAAA50.1072271070126528No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1072271070126528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1072271070126528No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG50.1072271070126528Illumina PCR Primer Index 7 (96% over 27bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1072271070126528No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATTCCGT50.1072271070126528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGGG100.0059351632149.4857270
GGTGAAA156.351648E-493.4285757
GTGAAAA351.8364517E-884.61455558
AGGGGGG802.5465852E-1174.7428670
GCGTGAA200.001991546770.0714356
CGTGAAA200.001991546770.0714357
TGAAAAA1100.058.33276459
AAGGGGG907.039489E-1052.8484869
GGGGGGG1302.5465852E-1151.74505670
AAAGGGG901.0859367E-950.55072468
GCTTGAA653.0409865E-850.3076956
CTTGAAA653.0409865E-850.3076957
TTTAAAA300.00793318949.35849458
CTTATAC1150.048.9200551
AAAAGGG901.6443664E-948.44444367
AAAAAGG901.6443664E-948.44444366
CGTCTTC851.4006218E-1046.16470748
TTGAAAA755.9097147E-846.06792458
CTGCTTG601.3081699E-645.8947454
TTCTGCT706.33263E-845.89473752
Produced by FastQC (version 0.11.5)