FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004137
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27420.27307030813524447No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25230.25126053516601815No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23100.23004829022334602No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21120.21032986534705922No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19660.19579001670090834No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17470.17398024373168203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16770.16700908342188367No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16480.16412103129353864No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16370.16302556324485604No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15190.1512741787226245No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14470.14410384240397475No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13250.13195410586404047No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12980.1292652297445468No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12480.12428582952326225No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12310.12259283344802552No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11990.1194060173064034No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11980.11930642930197773No Hit
CAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT11950.11900766528870063No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11720.11671714118690975No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG11380.11333114903643625No Hit
ATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT11370.11323156103201057No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA11070.11024392089923984No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10360.1031731725850158No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAA10280.10237646854961027No Hit
ACATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAA10190.10148017650977906No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT10080.10038470846109646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)