FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600540
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGA65560.40961175603233907No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGC61270.38280830219800815No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGA55770.3484448998463019No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53000.33113824084371524No Hit
GGTTATGATGTCCACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGG50540.3157684281554975No Hit
GTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG45310.28309195646469315No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC44950.28084271558349055No Hit
GTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC42320.26441076136803826No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41890.26172416809326854No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40520.25316455696202533No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38510.2406062953753108No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37010.2312344583703No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33480.20917940195184126No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33230.20761742911767278No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32560.20343134192210127No Hit
GTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG32050.2002449173403976No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31050.19399702600372376No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG30780.19231009534282179No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT30740.19206017968935485No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29740.18581228835268096No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28050.17525335199370212No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27910.1743786472065678No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAG27030.16888050283029477No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26010.16250765366688744No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC25910.16188286453322004No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25690.1605083284391518No Hit
TCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCGTCAGGGGTCCCTGACCGCTTCTCTGG25420.15882139777824983No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25010.15625976233021355No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24690.15426043710247792No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGAC24400.15244854861484247No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24230.15138640708760792No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG24180.15107401252077424No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23770.14851237707273796No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT23340.14582578379796818No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC22600.14120234420882952No Hit
GCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCT22570.1410149074687293No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT22530.14076499181526236No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21860.13657890461969086No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21430.13389231134492108No Hit
TCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCCAGAGAAGCGGTCAGGGACC21360.1334549589513539No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21190.13239281742411935No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGG21080.13170554937708523No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA21030.13139315481025154No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20910.13064340784985068No Hit
GCCTCATCCTCAGCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCC20880.13045597110975046No Hit
GTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCC20720.12945630849588263No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTA20640.12895647718894873No Hit
GTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG20600.12870656153548177No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC20460.12783185674834743No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGT20110.12564509478051158No Hit
GATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGG20050.12527022130031115No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19610.12252114911217465No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19610.12252114911217465No Hit
GTCTGGCGCCTCAGCCTCCCTGGCCGTCACTGGGCTTCAGGCTGAGGATG19590.12239619128544116No Hit
GGATGAGGCTGATTATTCCTGCCAGTCCTATGACAACAGTCTGGGTGCCTGGGTGTTCGGCGGAGGGACCAAG19420.12133404975820661No Hit
GTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAG19130.11952216127057119No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19020.11883489322353706No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT18890.11802266734976946No Hit
GCCTGAAGCCCAGTGACGGCCAGGGAGGCTGAGGCGCCAGACTGGGAGCC18740.11708548364926838No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG18230.1138990590675647No Hit
AGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGG18190.11364914341409774No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC18140.11333674884726405No Hit
CTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGG17980.11233708623339624No Hit
GCTTGGTCCCTCCGCCGAACACCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCT17830.11139990253289514No Hit
GTCAGGGACCCCTGACGGCCGATTGTTGTTACCATAGATGACGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA17760.11096255013932797No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17590.10990040861209342No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17420.10883826708485886No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17230.10765116773089083No Hit
GCTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAGCTTCCA17150.10715133642395691No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16980.10608919489672236No Hit
GGGTCCTGGGCCCAGTCTGTACTGGCGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC16800.10496457445612105No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16680.10421482749572018No Hit
CCCAGGCACCCAGACTGTTGTCATAGGACTGGCAGGAATAATCAGCCTCATCCTCAGCCTGAAGCCCAGTGACGG16480.10296524922838542No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16220.10134079748085022No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG16210.10127831856748348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)