FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954664
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT40760.2085268874855218No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT38930.19916466461755064No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38180.19532768803231654No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37000.18929084487154826No Hit
GTTCCAACACAGACCAGGGAAAGCCCCTAAGCTCCTGATCTACGGTTCTT36080.1845841535936611No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC33540.17158959289166834No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31880.16309708471635023No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31860.16299476534074397No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30820.1576741578092194No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30620.15665096405315695No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30540.156241686550732No Hit
GTGTTGGAACCAATTTAAATACTTGCTAATGTCATGACTCGCCTGGCAAG29890.1529163068435291No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29740.1521489115264823No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27880.14263320959510178No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACGGTTCTTCCAATTTGGAA27370.14002406551714258No Hit
GTCATAGTGCTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGC26560.13588013080508976No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG26460.13536853392705855No Hit
CAATAATACACGGCCGTGTCCTCGGCTGTCAGGTTGTTCATTTGCAGAAA26430.1352150548636492No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25840.13219663328326506No Hit
CTATGACAGTCTCCCTTTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25510.13050836358576207No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG24990.12784805981999975No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24980.12779690013219663No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCACGTCATCCAATAGGTAC24760.12667138700052796No Hit
GTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG24370.12467615917620624No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT23800.12176005697142835No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC23730.12140193915680648No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23580.12063454383975968No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22930.11730916413255679No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22930.11730916413255679No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTTAAAGAAAAATCTGTCCCAGATCCACTTCCA22350.11434190223997577No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22340.11429074255217264No Hit
ATAATACACGGCCGTGTCCTCGGCTGTCAGGTTGTTCATTTGCAGAAACA22110.11311406973270087No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21780.11142580003519785No Hit
ATATTACTGTCAGCACTATGACAGTCTCCCTTTTACTTTCGGCGGAGGGA21720.11111884190837915No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTA21600.11050492565474168No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21520.11009564815231672No Hit
CTGTCATAGTGCTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTTAAAGAAAAATCTGTC21430.10963521096208863No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA21400.10948173189867925No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21300.10897013502064806No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20520.1049796793720046No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19880.10170545935260485No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19780.10119386247457363No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT19780.10119386247457363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5250.041.956671
TGGTATC5500.040.6430662
GGTATCA14550.037.3744161
CGCTAAT655.53473E-737.08407232
GTATCAA28900.035.8466841
TAAGCGT400.002605715834.4758336
TCGTATG751.6564591E-632.2336540
TATCAAC34700.029.7322372
ATCAACG35900.028.8341983
TCAACGC36300.028.5164644
CGTATGC854.3768196E-628.45607641
CAACGCA37700.027.822385
TTAACGG1254.2055035E-927.56935135
TTCGGGG18400.027.48309570
AACGCAG38050.027.3856966
GCTAATC1306.4192136E-926.49676733
TACAGAT15350.025.76382370
AGAGTAC40500.025.47689411
GTAGTTT3351.0913936E-1125.15865570
CAGAGTA41550.024.99862710
Produced by FastQC (version 0.11.5)