FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180708
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC40480.3428451403734031No Hit
CTATAATACTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT37720.31946933534794375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25310.21436290767912133No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19380.16413880485268162No Hit
GAGTATTAGTAGGTGGTTGGCGTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCATGATCTATAAGGCGT18370.1555846153324954No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16860.14279567852508834No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16600.1405936099357335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15740.13330984460171355No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15210.1288210124772594No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15120.12805875796555966No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14910.12628016410492687No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13970.1183188392049516No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13590.11510043126666374No Hit
GTCTCTGGTGTCTCCATCAGTAGTGAAGAGTTCTACTGGAGTTGGATCCG13380.11332183740603095No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12950.10967995473902098No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCAGTTGTCGGGCCAGTCAGA11980.10146454500181247No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11890.10070229049011271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23600.040.8837851
TATCAAC26450.035.9498022
ATCAACG27650.034.2649963
TCAACGC27950.033.8972134
CAACGCA28500.033.122175
AACGCAG29400.032.2281386
AGAGTAC31800.030.1451311
CGCAGAG31800.029.9269898
ACGCAGA32300.029.3482637
TATTCCG604.9092266E-429.24240364
GCAGAGT33300.028.6824769
AGTACTT8650.027.10767613
GAGTACT9050.026.29057112
AATAGCG1358.090865E-925.92503262
AGTAACG700.001335822424.63666223
AGTACGG29950.024.17808713
GAGTACG30150.024.01770412
TATAAGA1902.7284841E-1123.5723482
TACTTTT10100.023.552415
TCAAACG13700.022.77925745
Produced by FastQC (version 0.11.5)