FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996811
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32240.32343142280733256No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG27610.27698329974288005No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24240.24317548662685304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23720.23795885077512186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22330.22401438186376355No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAA22170.22240926314015397No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22140.22210830337947718No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21790.21859710617158118No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21600.21669102768729478No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21560.2162897480063924No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21380.21448398944233157No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21260.21328015039962442No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATG20880.20946799343105166No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20710.20776255478721645No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20580.20645839582428363No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCAC20360.20425135757932045No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20090.20154271973322926No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20080.20144239981300366No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18730.18789921058254774No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATTACTGAACACAGA18190.18248193489036538No Hit
ATATACACACTATGCAGCCTCCGTGAAGGACCGCTTCACCATCTCCAGAG18050.18107745600720698No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18010.1806761763263046No Hit
AGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAACCAGGGAA17700.177566258799311No Hit
GTGTGTATATCTTCCATCAAAAGAGATGACTGCCACCCACTCCGGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG17640.1769643392779574No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT17380.17435602135209183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17020.17074450422397022No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16310.16362178988795267No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16240.1629195504463735No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA16220.16271891060592228No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16220.16271891060592228No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15670.15720131499351434No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCCGGAGTGGGTGGC15620.1566997153923863No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15280.15328883810471594No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG15220.15268691858336234No Hit
GCCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC15100.15148307954065515No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14820.14867412177433836No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14590.14636676360914957No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14340.14385876560350958No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA14300.14345748592260718No Hit
CTATGCAGCCTCCGTGAAGGACCGCTTCACCATCTCCAGAGACAATTCCAGGAACACTCTGTATTTGGAAGTCAA14250.1429558863214792No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14220.1426549265608024No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATGAGGGGGAGA14150.1419526871192232No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13930.13974564887426003No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13630.13673605126749203No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13530.13573285206523603No Hit
CCCTAACACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGGGGCTATGCT13410.13452901302252884No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13210.13252261461801684No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13100.13141909549553527No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13010.13051621621350487No Hit
GTATTTGGAAGTCAACAGCCTCAGAGGTGACGACACGGGTGTATATTTCT12840.12881077756966966No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12740.1278075783674137No Hit
TTACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12370.1240957413190665No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12270.1230925421168105No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12190.1222899827550057No Hit
ATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT12170.1220893429145545No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12040.12078518395162172No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC11970.12008294451004253No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11930.11968166482914014No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11810.11847782578643293No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11750.11787590626507934No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11630.11667206722237214No Hit
GCCCAGCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATTACTGAAC11620.11657174730214655No Hit
CCCTGGTCCCTCCGCCGAAAATGAGGGGGAGACTGCCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGG11450.11486630865831136No Hit
ATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGA11380.11416406921673217No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11360.11396342937628097No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11340.11376278953582977No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11320.11356214969537858No Hit
GACCAGGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT11310.11346182977515296No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11200.11235831065267138No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11140.11175639113131777No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGGGGCTATGCTATGCACTGGG11100.11135511145041538No Hit
CAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT11090.11125479153018979No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11080.11115447160996417No Hit
ACCTTGATGGGACCCCTGCTTCCAAGTCGGAGGCATCGAAAATCAGGAGC10730.10764327440206818No Hit
ACATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAA10720.1075429544818426No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT10660.106941034960489No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10630.10664007519981221No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG10600.10633911543913539No Hit
GAAATATACACCCGTGTCGTCACCTCTGAGGCTGTTGACTTCCAAATACA10600.10633911543913539No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10600.10633911543913539No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10540.1057371959177818No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10510.10543623615710501No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA10490.10523559631665381No Hit
ACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCT10460.104934636555977No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC10390.10423239711439782No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10390.10423239711439782No Hit
ATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCCGGAGTGGGTG10240.10272759831101383No Hit
GGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10180.10212567878966022No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG10130.10162407918853224No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10120.10152375926830662No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAG10100.10132311942785542No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC10090.10122279950762983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)