FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996811
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32190.3229298232062046No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG27920.28009321726987363No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25900.25982859338430253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21070.21137407191533802No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20990.21057151255353324No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20820.20886607390969802No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20320.20385007789841805No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATG20220.20284687869616208No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20020.20084048029165008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19970.20033888069052205No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAA19880.1994360014084917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19780.1984328022062357No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19430.19492160499833971No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCGCAC18890.18950432930615735No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18620.18679569146006617No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18320.18378609385329817No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18070.1812780958476582No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATTACTGAACACAGA17970.1802748966454022No Hit
GTGTGTATATCTTCCATCAAAAGAGATGACTGCCACCCACTCCGGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG17930.1798736169644998No Hit
AGTCAGGACATTCGCACCTCTTTAAATTGGTATCAACAAAAACCAGGGAA17770.1782684982408902No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17380.17435602135209183No Hit
ATATACACACTATGCAGCCTCCGTGAAGGACCGCTTCACCATCTCCAGAG17140.17194834326667743No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16350.16402306956885507No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16350.16402306956885507No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16280.16332083012727588No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16240.1629195504463735No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15240.15288755842381355No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT15130.15178403930133194No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA14890.14937636121591755No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14340.14385876560350958No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCCGGAGTGGGTGGC14210.14255460664057681No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14210.14255460664057681No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14180.1422536468799No Hit
GCCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC13920.13964532895403442No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC13730.13773925046974803No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13710.13753861062929681No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13430.13472965286298005No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG13430.13472965286298005No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTCCCAGATCCACGTCCA13420.13462933294275445No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13390.13432837318207766No Hit
CTATGCAGCCTCCGTGAAGGACCGCTTCACCATCTCCAGAGACAATTCCAGGAACACTCTGTATTTGGAAGTCAA13310.13352581382027284No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCCGCCGAAAATGAGGGGGAGA13160.13202101501688887No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13110.13151941541576084No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13070.13111813573485845No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12920.12961333693147448No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12870.12911173733034648No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12810.12850981780899287No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12770.12810853812809048No Hit
GGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12750.1279078982876393No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12660.1270050190056089No Hit
CCCTAACACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGGGGCTATGCT12490.12529958036177372No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12250.12289190227635931No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12180.12218966283478012No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12160.12198902299432891No Hit
CTGCCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGAAAAATCAGTC12120.12158774331342652No Hit
ATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCAT12050.12088550387184734No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12020.12058454411117052No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11860.11897942538756093No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11780.11817686602575614No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11720.11757494650440255No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT11700.11737430666395135No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11670.11707334690327453No Hit
TTACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC11660.11697302698304894No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11600.11637110746169535No Hit
CCCTGGTCCCTCCGCCGAAAATGAGGGGGAGACTGCCATATTGTTGACAGTAATATGTTGCAAAATCTTCAGG11500.11536790825943935No Hit
GCCCAGCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATTACTGAAC11500.11536790825943935No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11440.11476598873808574No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGGGGCTATGCTATGCACTGGG11390.11426438913695776No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11360.11396342937628097No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11190.11225799073244576No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11080.11115447160996417No Hit
GTATTTGGAAGTCAACAGCCTCAGAGGTGACGACACGGGTGTATATTTCT11000.11035191224815938No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10970.11005095248748259No Hit
GAAATATACACCCGTGTCGTCACCTCTGAGGCTGTTGACTTCCAAATACA10760.107944234162745No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10760.107944234162745No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC10750.10784391424251939No Hit
ATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGA10650.1068407150402634No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10620.1065397552795866No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10530.10563687599755621No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10440.10473399671552583No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG10440.10473399671552583No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10340.10373079751326983No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT10340.10373079751326983No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10290.10322919791214182No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10060.10092183974695303No Hit
ACATATTACTGTCAACAATATGGCAGTCTCCCCCTCATTTTCGGCGGAGGGACCAGGGTGGACATCAAACGAA9970.10001896046492263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1800.051.6742251
ATCAACG18000.036.1683273
CTCACAA901.4383398E-436.0424170
TCAACGC18250.035.4841274
CAACGCA18450.035.0994725
AACGCAG18950.034.3603136
CGCAGAG19350.033.6821258
CAGAGTA19700.033.08371410
AGAGTAC19750.032.99995411
GCAGAGT20100.032.425339
TTAACGG1101.036824E-931.42174735
CTTAGAC553.2867555E-431.314573
TCGAGTG450.00457283430.73431637
TATTCTA850.004722488630.53003970
ACGCAGA21400.030.4463947
CGCGGTA1151.6243575E-930.13445543
TTTAACG1202.6375346E-928.7974734
ATTAGAG1202.730303E-928.7050233
TATTAGA1202.730303E-928.7050232
TAACGGC1103.5899575E-828.28526736
Produced by FastQC (version 0.11.5)