FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255928
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTAACCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT118370.9424903338407934No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76970.6128536030727877No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC60230.4795657075883331No Hit
CTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGACTGCCGATGGTGAGAGTGAATTCTGTC57320.45639558955608917No Hit
GTAGTAACCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGA52750.4200081533336306No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52400.4172213693778624No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50840.40480027517501005No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG45960.36594454459172815No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT45560.3627596486422789No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43890.3494627080533279No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42880.3414208457809684No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42710.3400672650024524No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTTACAT42360.33728048104668423No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCATTTGCCGGGCCAGTGAGA42150.3356084106732233No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41730.3322642699263015No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40280.32071902210954767No Hit
GTAGGAGACAGAGTCACCATCATTTGCCGGGCCAGTGAGAGTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAA39240.3124382926409794No Hit
CCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATT38670.30789981591301413No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG38330.3051926543559822No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC38100.3033613391850488No Hit
CTCCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTT38080.30320209438757634No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36490.2905421329885153No Hit
GTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTATTA36290.2889496850137906No Hit
GTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTACACTCCTGATGTATAGGGCGTCTA36170.2879942162289558No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34850.277484059595773No Hit
GAATTCACTCTCACCATCGGCAGTCTGCAGCCTGATGATTTTGCGACTTATTACTGCCAACAGTATAGTAGTAAC32390.25789694950665965No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTCTCCGTCCACC32230.25662299112687986No Hit
CCCCTACACTCCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCA31780.2530399831837494No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA31750.2528011159875407No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31560.25128829041155226No Hit
CCGTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCATTTGCCG31380.24985508723430003No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31170.24818301686083916No Hit
GGTTACTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGACTGCCGATGGTGAGAGTGAATT30180.24030039938595207No Hit
ATACATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTATTAATACTCTCACTGGC30080.23950417539858973No Hit
CAGTATAGTAGTAACCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT29180.23233815951232872No Hit
CATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACC28270.22509252122733153No Hit
GATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCA28210.2246147868349141No Hit
GAGTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTACACTCCTGATGTATAGGGCGT28160.22421667484123295No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27620.21991706530947636No Hit
GTCTCCGTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCATTT27500.21896159652464156No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27080.21561745577771976No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27020.21513972138530235No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG26580.21163633584090807No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26150.20821257269525006No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25760.20510729914453696No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25750.2050276767458007No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25650.20423145275843838No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24960.1987375072456383No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24930.1984986400494296No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24890.19818015045448464No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24790.1973839264671223No Hit
GTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCACTCT24130.19212884815053094No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC23430.1865552802389946No Hit
ACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCACTCTCACCATCGGCAGT23340.1858386786503685No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC23140.18424623067564383No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21930.1746119204285596No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21030.1674459045422986No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20760.1652960997764203No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20530.16346478460548694No Hit
GTCTGCAGCCTGATGATTTTGCGACTTATTACTGCCAACAGTATAGTAGTAACCCGTACACTTTTGGCCAGGGGA20430.16266856061812462No Hit
CCCTATACATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTATTAATACTCTCA20270.16139460223834487No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19850.15805046149142307No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19380.1543082087508201No Hit
CCTATTAATACTCTCACTGGCCCGGCAAATGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGACGGAGA19320.15383047435840272No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19150.15247689357988672No Hit
GCCCCTACACTCCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCT18780.14953086482664613No Hit
GCTCTGGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTCTCCGTCCACCCTGTCTGCATCTGTAGGAGA18740.14921237523170117No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18570.14785879445318523No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18440.1468237032696142No Hit
GGTATCAGCAGAAACCAGGGAGAGCCCCTACACTCCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCAT18430.14674408087087795No Hit
TGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCACT18020.1434795625226924No Hit
GGCCAAAAGTGTACGGGTTACTACTATACTGTTGGCAGTAATAAGTCGCA17980.14316107292774746No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17830.14196673694670395No Hit
ATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTAC17780.14156862495302278No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17610.14021504417450684No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17500.13933919778840825No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17250.13734863782000242No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16830.13400449707308063No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16680.13281016109203714No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16630.13241204909835597No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTC16450.13097884592110376No Hit
GGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTCTCCGTCCACC16390.13050111152868638No Hit
CTATACATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGC16300.12978450994006024No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16280.1296252651425878No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16130.1284309291615443No Hit
CATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCACTCTCACCATCGGCAGTCTGCAGCCTGATGATTTTG16030.12763470517418196No Hit
ATTAATACTCTCACTGGCCCGGCAAATGATGGTGACTCTGTCTCCTACAG15710.12508678841462248No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15500.12341471804116158No Hit
ATGTTACATCCAGATGACCCAGTCTCCGTCCACCCTGTCTGCATCTGTAG15390.12253887165506303No Hit
GTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTCT15340.12214075966138185No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTAGACGCCCTATACATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTG15160.12070755648412967No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15150.12062793408539342No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15000.11943359810434995No Hit
CCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGG14890.11855775171825135No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14840.1181596397245702No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14790.11776152773088903No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14730.11728379333847162No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14690.1169653037435267No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14500.11545247816753827No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14420.11481549897764841No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14410.11473587657891215No Hit
CACCATCGGCAGTCTGCAGCCTGATGATTTTGCGACTTATTACTGCCAAC14230.11330267340165998No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14080.11210833742061647No Hit
GTTACATCCAGATGACCCAGTCTCCGTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCATTTGCCGGG14040.11178984782567153No Hit
ACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGACTGC14040.11178984782567153No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC13880.11051588944589179No Hit
GTCTCCTACAGATGCAGACAGGGTGGACGGAGACTGGGTCATCTGGATGTAACATTTGGCACCTGGGAGCCAGAG13840.11019739985094687No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13790.1097992878572657No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13380.10653476950908014No Hit
CTGTAGGAGACAGAGTCACCATCATTTGCCGGGCCAGTGAGAGTATTAAT13160.104783076736883No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12930.10295176156594965No Hit
CCCAGTCTCCGTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATC12840.10223515997732353No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12800.10191667038237862No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12650.1007223344013351No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12640.10064271200259887No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12570.10008535521144524No Hit
GAGTCACCATCATTTGCCGGGCCAGTGAGAGTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAGAG12570.10008535521144524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)