FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255928
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGACTGCCGATGGTGAGAGTGAATTCTGTC125941.002764489684122No Hit
GTATAGTAGTAACCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT110870.8827735347886185No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77110.6139683166550949No Hit
GGTTACTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGACTGCCGATGGTGAGAGTGAATT61100.48649285627838534No Hit
GTAGTAACCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGA50190.3996248192571549No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49800.39651954570644177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48920.38951277461765327No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC45120.3592562630978846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45010.358380416711786No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43770.3485072392684931No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43080.343013293755693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42640.3395099082112987No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37610.29945984164697337No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT37170.29595645610257915No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG35080.27931537476670637No Hit
GTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTATTA34750.27668783560841065No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG34340.2734233172602251No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33340.26546107738660174No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTTACAT32210.25646374632940744No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA31870.2537565847723755No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31400.25001433203177253No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30750.24483887611391736No Hit
GAATTCACTCTCACCATCGGCAGTCTGCAGCCTGATGATTTTGCGACTTATTACTGCCAACAGTATAGTAGTAAC30550.24324642813919267No Hit
GGCCAAAAGTGTACGGGTTACTACTATACTGTTGGCAGTAATAAGTCGCA30480.24268907134803908No Hit
CTCCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTT30010.23894681860743608No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29850.23767286022765635No Hit
ACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGACTGC29380.2339306074870534No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCATTTGCCGGGCCAGTGAGA28700.22851628437298954No Hit
ATACATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTATTAATACTCTCACTGGC28580.22756081558815475No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28540.2272423259932098No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28190.22445554203744164No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28050.22334082845513437No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27650.22015593250568505No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27440.21848386213222412No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26940.21450274219541246No Hit
CCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATT26870.21394538540425884No Hit
CAGTATAGTAGTAACCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26630.21203444783458925No Hit
CATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACC25900.20622201272684423No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25210.20072806721404413No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24960.1987375072456383No Hit
GTAGGAGACAGAGTCACCATCATTTGCCGGGCCAGTGAGAGTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAA24840.1977820384608035No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC24820.19762279366333102No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24350.19388054092272805No Hit
GAGTGAATTCTGTCCCAGAGCCGCTGCCGCTGAACCTTGATGGGACCCCA24220.19284544973915702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24110.19196960335305846No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23760.18918281939729029No Hit
GTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTACACTCCTGATGTATAGGGCGTCTA23340.1858386786503685No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23170.18448509787185255No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTCTCCGTCCACC22850.18193718111229304No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22450.17875228516284372No Hit
CCCCTACACTCCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCA22430.17859304036537127No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21690.17270098285889No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21120.1681625061309247No Hit
GTGTACGGGTTACTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATC21040.1675255269410348No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20840.16593307896631018No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20740.16513685497894784No Hit
CCGTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCATTTGCCG20170.16059837825098255No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20070.1598021542636202No Hit
GGCTGCAGACTGCCGATGGTGAGAGTGAATTCTGTCCCAGAGCCGCTGCC19940.15876706308004918No Hit
GTCTGCAGCCTGATGATTTTGCGACTTATTACTGCCAACAGTATAGTAGTAACCCGTACACTTTTGGCCAGGGGA19830.15789121669395062No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC19740.15717461510532452No Hit
GATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCA19730.15709499270658828No Hit
GTCCCAGAGCCGCTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAAGT18690.14881426323802002No Hit
CCCTATACATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTATTAATACTCTCA18150.14451465370626343No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17960.14300182813027498No Hit
GTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCACTCT17800.14172786975049526No Hit
ACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCACTCTCACCATCGGCAGT17490.139259575389672No Hit
CCTATTAATACTCTCACTGGCCCGGCAAATGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGACGGAGA17470.13910033059219953No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC17340.1380652394086285No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17240.13726901542126618No Hit
CCTTGGTCCCCTGGCCAAAAGTGTACGGGTTACTACTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGG17180.13679128102884877No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17170.13671165863011256No Hit
GTCTCCGTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCATTT17110.13623392423769515No Hit
GAGTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTACACTCCTGATGTATAGGGCGT16980.1351988330541241No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16710.13304902828824583No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16710.13304902828824583No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16670.13273053869330087No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16560.13185469230720231No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16500.13137695791478493No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16460.13105846831983997No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16300.12978450994006024No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16220.12914753075017038No Hit
GTAATAAGTCGCAAAATCATCAGGCTGCAGACTGCCGATGGTGAGAGTGAATTCTGTCCCAGAGCCGCTGCCG16190.1289086635539617No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15830.1260422571994573No Hit
CTATACATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGC15500.12341471804116158No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15490.12333509564242537No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15310.12190189246517316No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14920.11879661891446007No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14550.11585059016121944No Hit
ATTAATACTCTCACTGGCCCGGCAAATGATGGTGACTCTGTCTCCTACAG14320.11401927499028607No Hit
GCCCCTACACTCCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCT14210.11314342860418751No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14080.11210833742061647No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13980.11131211343325413No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCACT13940.11099362383830921No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13740.10940117586358453No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13700.10908268626863961No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13590.10820683988254104No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13550.10788835028759611No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13520.1076494830913874No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTAGACGCCCTATACATCAGGAGTGTAGGGGCTCTCCCTGGTTTCTG13520.1076494830913874No Hit
CATCAAGGTTCAGCGGCAGCGGCTCTGGGACAGAATTCACTCTCACCATCGGCAGTCTGCAGCCTGATGATTTTG13470.10725137109770623No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13420.10685325910402507No Hit
GGTATCAGCAGAAACCAGGGAGAGCCCCTACACTCCTGATGTATAGGGCGTCTACTTTACAAAGTGGGGTCCCAT13380.10653476950908014No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13350.10629590231287145No Hit
CACCATCGGCAGTCTGCAGCCTGATGATTTTGCGACTTATTACTGCCAAC13320.10605703511666273No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTC13220.10526081112930041No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13200.10510156633182793No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13110.10438496474320184No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13090.10422571994572938No Hit
GTCTCCTACAGATGCAGACAGGGTGGACGGAGACTGGGTCATCTGGATGTAACATTTGGCACCTGGGAGCCAGAG13070.1040664751482569No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12960.10319062876215834No Hit
GCTCTGGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTCTCCGTCCACCCTGTCTGCATCTGTAGGAGA12950.10311100636342212No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12810.10199629278111483No Hit
CCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGG12740.10143893598996122No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC12700.10112044639501627No Hit
GTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTTACATCCAGATGACCCAGTCT12680.10096120159754379No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12620.10048346720512641No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12570.10008535521144524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)