FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534614
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56220.3663461951995746No Hit
GTTTTAAACAGCTCCAACAATAAGAATTCCGTAGCTTGGTACCAGCAGAA44970.2930378583800226No Hit
ATACTATAGTCCACCGTACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCAT43010.28026591703190507No Hit
CTATAGTATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTT42670.2780503761858031No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38740.25244133052350626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36970.24090748553056338No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35270.22982978130005333No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35220.22950396646974416No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34240.22311799579568542No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32950.21471197317371013No Hit
ATTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTACGGAATTCTTATTGTTGGAGCTGTTTAAAACACT31940.2081305136014659No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC31760.20695758021235308No Hit
CTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCA29690.1934688462375555No Hit
GGCCAAAAGTGTACGGTGGACTATAGTATTGCTGACAGTAATAAACTGCC29110.18968939420596972No Hit
GTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCAC28810.187734505224115No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCCCCTGGCCAAAAGTG28360.18480217175133293No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCA27650.1801756011609434No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAGGTCCAGTAAATGAGCAATTTAGGAGGCTGTCCTGGTTTCTGCTGGTA27480.1790678307378924No Hit
CTCCTAAATTGCTCATTTACTGGACCTCTACCCGGGAATCCGGGGTCCCT27100.1765916380275431No Hit
GTTTAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCGTCTCGCCCAGCACCACAGCCAGGAAGTCTGGAGC27090.17652647506148125No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTTCCAGACCCGCTGCCACTGAAGCGGTCAGGGACC26140.17033599328560797No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26000.16942371176074245No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25150.1638848596454874No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24650.1606267113423962No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24580.16017057057996345No Hit
GTGCTGACCCAGGCTCCAGACTTCCTGGCTGTGGTGCTGGGCGAGACGGC24500.15964926685146885No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24240.1579550297338614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23540.15339362210953375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22800.14857156262095875No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTACGGAATTCTTATTGTTGGAGCTG22580.14713797736759862No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22460.14635602177485674No Hit
GGACTATAGTATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22360.1457043921142385No Hit
AACCAGGACAGCCTCCTAAATTGCTCATTTACTGGACCTCTACCCGGGAA22120.14414048092875473No Hit
GTGCTGGGCGAGACGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTT21900.1427068956753946No Hit
CTTCAGTGGCAGCGGGTCTGGAACAGATTTCACTCTCACCATCAGCAGCC21690.1413384733880963No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG21540.14036102889716892No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21130.13768934728863416No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20850.13586478423890305No Hit
GTTTATTACTGTCAGCAATACTATAGTCCACCGTACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAA20700.13488733974797573No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20630.13443119898554293No Hit
GTTCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGATTCCCGGGT20160.1313685395806372No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20120.13110788771638993No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19950.1300001172933389No Hit
CTATAGTCCACCGTACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT19800.12902267280241156No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19440.1266768060241859No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19030.1240051244156511No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18790.12244121323016734No Hit
GTGGACTATAGTATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT18670.12165925763742544No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18620.1213334428071163No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18390.11983469458769437No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18250.11892241306282883No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGCTGACCCAGGCTCCAGACTTCCTGGCTGTGGTGCTGGGCGAGACGG18140.11820562043614877No Hit
ATAGTCCACCGTACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGA17730.11553393882761398No Hit
ATAGTATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGC17620.11481714620093392No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17570.11449133137062481No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17250.11240611645664642No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17210.11214546459239914No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16920.11025573857660624No Hit
GGGCGAGACGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTAAACAGCTCCAACAATAAGAATTCCGTAG16480.10738856806988598No Hit
GAGTGAAATCTGTTCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCG15930.10380460493648565No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15700.10230585671706371No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATACTATAGTCCACCGT15670.10211036781887822No Hit
GTGTACGGTGGACTATAGTATTGCTGACAGTAATAAACTGCCACATCCTC15650.10198004188675458No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15640.10191487892069276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)