FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838387
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47930.26071768349101687No Hit
GTATGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC43050.23417267419754384No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33730.18347605808787812No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33040.17972276783941574No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCA31940.17373926164621487No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30600.16645026319267925No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30400.16536235297573362No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCCG30090.16367609213946793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30050.16345851009607879No Hit
GTCTCCACTCTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCATA29010.1578013769679616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28870.15703983981609965No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC28790.1566046757293214No Hit
GTCTGGGACCCCAGAGTCCCGGTGAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGATGAAA27910.1518178707747607No Hit
GTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCACTGATTTCACACTGAA26090.1419178878005556No Hit
GTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACACGAACTGTGGCTGCACCAT25610.1393069032798861No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25320.13772943346531497No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTG24670.1341937252602417No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24520.1333777925975325No Hit
GGTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGA24050.13082120358771032No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGT23850.1297332933707647No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23570.12821021906704083No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23170.1260343986331496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22930.12472890637281486No Hit
GTATTATAGTGGGCTCACCCACTACAAGCCGTCCCTCCAGAGTCGAGTCA22770.12385857819925837No Hit
GTGATGGAAACACCTTCTTGAATTGGTTTCATCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT21910.11918056426639223No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21690.11798386302775205No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20640.11227233438878756No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTCCACTCTCC20520.1116195882586202No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20420.11107563315014739No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTGAGCCCCGGAGG20400.11096684212845281No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20350.11069486457421641No Hit
GCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTGAGCCCCGGAGGCCAGTATGAACCTTGCATG20290.11036849150913274No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20250.1101509094657436No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTCCACT20180.10977014088981264No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20150.1096069543572708No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAACTTCAGGGAGAGTTCCTTCTTGGACGTGTCCACGGC19190.10438498531593185No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18890.10275311999051343No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18690.10166520977356781No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGA18600.10117565017594228No Hit
CTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA18560.10095806813255315No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18450.10035971751323307No Hit
ATGTATTATAGTGGGCTCACCCACTACAAGCCGTCCCTCCAGAGTCGAGT18440.10030532200238579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)