FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831369
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGCTGTCGTCATAGGA29920.35988832876857324No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC28960.3483411096636993No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGG27050.32536695498629364No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20330.24453642125217565No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18440.22180283363945494No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT17160.20640654149962293No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16800.20207633433529515No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16420.19750556010628254No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16010.19257393528024258No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15330.18439465508095682No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15260.18355267035455977No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTG14600.17561395721995887No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14210.1709228994586038No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13790.16587099110022144No Hit
ATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG13630.16394645458274246No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13530.16274361925931807No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13040.15684972617453863No Hit
GTCCTATGACGACAGCCTGAGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG12880.15492518965705962No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12670.15239923547786843No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT12580.1513166836867865No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGC12530.15071526602507432No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12010.14446052234326753No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12000.14434023881092511No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11930.14349825408452804No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11850.14253598582578855No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11680.14049116577596713No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC11520.13856662925848812No Hit
TCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGT11360.1366420927410091No Hit
CCCAGGCACTCAGGCTGTCGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGG11230.13507840682055744No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTATGATGTACACTGGTACCAG11210.13483783975587255No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT11080.13327415383542085No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG10630.12786139488001116No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC10570.12713969368595654No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG10450.1256962912978473No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10140.12196750179523173No Hit
CGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAGCAATCGG9970.11992268174541028No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG9850.11847927935730103No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9570.11511134045171277No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC9400.11306652040189134No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT9300.11186368507846696No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9190.11054056622270016No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8930.10741319438179676No Hit
GATTATTACTGCCAGTCCTATGACGACAGCCTGAGTGCCTGGGTGTTCGG8860.1065712096553997No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT8850.10645092612305727No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT8760.10536837433197534No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG8720.10488724020260559No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8670.1042858225408934No Hit
GCTCCAACATCGGGGCACGTTATGATGTACACTGGTACCAGCAGCTTCCA8640.10392497194386609No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8580.10320327074981146No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8550.10284242015278415No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8520.10248156955575682No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC8480.10200043542638708No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8460.10175986836170221No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8430.10139901776467489No Hit
GTCGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCC8410.10115845069999002No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8360.10055703303827783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC452.9484E-853.8462141
TCGTATG452.9611329E-853.8169540
GTGGTAT2050.047.1400871
GGTATCA7350.046.4872741
CGTACTA551.4730176E-743.89426829
ACCGTAC551.4758189E-743.88370527
TGGTATC2300.041.9656222
ACTAGAT557.375478E-637.6485832
ACGAGAC655.486454E-737.12120422
GTATCAA16200.036.6437261
TACTAGA601.3402248E-534.50704631
TATCAAC18450.032.1362762
TCAACGC19300.030.7209454
ATCTCGT450.004588854530.7117637
GATTGAC450.004613267330.678471
CCGTACC450.00464187930.6397224
CAACGCA19500.030.405865
ATCAACG19450.030.3067913
GTACTAG802.7599253E-630.1882130
AACGCAG20400.029.2334066
Produced by FastQC (version 0.11.5)