FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148153
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.048.013141
GTATCAA36350.041.8010061
TATCAAC42750.035.196152
TCGTATG709.521991E-734.59826740
ATCAACG43400.034.5896843
TCAACGC43450.034.549884
CAACGCA43500.034.431025
AACGCAG45450.033.1810466
ACGCAGA47250.031.7698847
CGCAGAG47500.031.6751568
AGAGTAC48400.031.1572911
CAGAGTA48900.030.69789310
ACGGGTA2550.029.70389716
GCAGAGT51100.029.3762599
TTAACGG4300.028.90148535
TAACGGC4550.028.07587836
CGCGGTA4400.027.5877543
ACCGTAT500.00781248827.5436138
AGTACGG41850.026.81964913
GTGGTAT4800.026.5590821
Produced by FastQC (version 0.11.5)