FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148153
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12240.10660600111657592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.042.349621
GTATCAA36900.041.831141
TATCAAC42550.035.7840352
TCAACGC43000.035.409554
ATCAACG43750.034.881093
CAACGCA44550.034.177575
AACGCAG45550.033.7320026
GTGGTAT5550.032.8293721
ACGCAGA48050.031.913767
CGCAGAG48250.031.645858
AGAGTAC48900.031.29688811
CAGAGTA49500.031.12596510
TGGTATC5800.030.814882
GCAGAGT50900.030.1347149
AGTACGG40850.027.1932113
GAGTACG41500.026.85016312
GCCGATT1458.2725215E-526.42919570
TACGGGT8100.024.20136515
GTACGGG45450.023.98592214
TACGGGG19250.023.58268215
Produced by FastQC (version 0.11.5)