FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218706
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGTACAGCGTCCCCAATCACCTTTAGCAACTATGCCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG46360.3804034771306615No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24900.20431506860555376No Hit
GTATATTACTGTGCGAAAGACCGAGGTCACTATGATAGTAGTGGCCTTTA23660.19414034229748603No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23360.191678714964889No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22610.1855246466333964No Hit
GTAATATACGGCCGTGTCTTCGGCTCGCAGGCTGTTCATTTGCAGATACA22520.1847861584336173No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21360.17526786608090877No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21310.17485759485880928No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21160.17362678119251077No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20940.17182158781527293No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20550.16862147228289678No Hit
CTATGATAGTAGTGGCCTTTATGATGCTTTTGACATTTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCCTC19400.15918523417460814No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18880.1549184134647733No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18340.15048748426609865No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17780.14589244657858416No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT16760.13752291364775426No Hit
GTACTGAAACTCTCTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT16740.13735880515891447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15700.12882516373924474No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTCGGCCGAACGTC15470.126937916117587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15370.12611737367338802No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15310.1256250482068686No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15110.12398396331847059No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCATAAACACGTTGTATCTGCAAATGAA15030.12332752936311137No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14530.11922481714211632No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14260.11700935254277899No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14260.11700935254277899No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13970.11462977945460184No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13960.11454772521018194No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13910.11413745398808244No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13600.11159377241106551No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT13360.10962447054498788No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACGGCCA13320.10929625356730828No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13240.10863981961194906No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13240.10863981961194906No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13200.10831160263426946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13000.10667051774587144No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12870.10560381256841272No Hit
CCTGTACAGCGTCCCCAATCACCTTTAGCAACTATGCCATGACTTGGGTC12770.1047832701242137No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCT12590.10330629372465548No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12520.10273191401371619No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12390.10166520883625747No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12360.10141904610299778No Hit
CTATCATAGTGACCTCGGTCTTTCGCACAGTAATATACGGCCGTGTCTTC12260.10059850365879877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA200.007346212451.7730132
GTGGTAT2850.042.366381
GTATCAA21650.039.5177271
TATCAAC24550.034.5473252
ATCAACG25950.032.816363
TCAACGC26050.032.6903844
CAACGCA26350.032.3181955
AACGCAG26900.031.9137526
AGAGTAC29750.028.97592411
CGCAGAG29750.028.9747358
ACGTGTT10500.028.8209751
TACAACG10250.028.78325847
CGTGTTT10550.028.69744552
ACAACGT10200.028.60060548
ATACAAC10350.028.49339146
AACGTGT10500.028.14327650
GCTATAC500.007760192727.5816863
GCCTAAT950.0089665526.77483670
CAGATAC11050.026.64657443
ACCGCAC1455.7298166E-1026.63921764
Produced by FastQC (version 0.11.5)