FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218706
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGTACAGCGTCCCCAATCACCTTTAGCAACTATGCCATGACTTGGGTCCGCCAGGCTCCAGGGAAGGGG45200.370885184777953No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25550.20964859449284734No Hit
GTAATATACGGCCGTGTCTTCGGCTCGCAGGCTGTTCATTTGCAGATACA24850.20390479738345424No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24330.1996379766736194No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22640.1857708093666561No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21690.1779756561467655No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21410.17567813730300827No Hit
GTATATTACTGTGCGAAAGACCGAGGTCACTATGATAGTAGTGGCCTTTA21130.17338061845925104No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19670.16140069877394547No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19580.16066221057416638No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18940.1554107389312927No Hit
CTATGATAGTAGTGGCCTTTATGATGCTTTTGACATTTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCCTC18450.15139008095471754No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17470.14334876500156724No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16510.13547155753725673No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT15980.13112268258300197No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTCGGCCGAACGTC15470.126937916117587No Hit
GTACTGAAACTCTCTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT15180.12455834302940988No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15090.12381985482963077No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15010.12316342087427157No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14680.12045563080841483No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCATAAACACGTTGTATCTGCAAATGAA14210.11659908132067946No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14160.11618881009857997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14120.11586059312090036No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13960.11454772521018194No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13850.11364512852156303No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13810.11331691154388343No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13790.11315280305504362No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13720.11257842334410433No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13510.11085528421128639No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13430.11019885025592718No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13370.10970652478940777No Hit
CTATCATAGTGACCTCGGTCTTTCGCACAGTAATATACGGCCGTGTCTTC13250.10872187385636896No Hit
CCTGTACAGCGTCCCCAATCACCTTTAGCAACTATGCCATGACTTGGGTC12990.10658846350145154No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCT12640.103716564946755No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA12630.1036345107023351No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12500.10256780552487638No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACGGCCA12490.10248575128045648No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12470.1023216427916167No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12400.10174726308067737No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12280.10076261214763856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22150.042.5762941
GTGGTAT3750.038.548641
TATCAAC25600.036.6995122
ATCAACG26500.035.453113
TCAACGC26600.035.3198284
CAACGCA26750.035.1217735
AACGCAG27150.034.8592646
AGAGTAC28950.032.47941211
CGCAGAG29350.032.152798
GTTCGAA450.004660702330.6155159
TGTTAGA4950.030.15246870
CAGAGTA31500.030.06880810
AGTACGG25000.029.75827813
GAGTACG25450.029.09676612
CGTGTTT10800.028.9937252
ACGTGTT10800.028.64542851
GTCTAGA500.00782496227.5347421
GTATAGC756.263986E-527.534741
TAAACGC500.00782966427.5313534
AACGTGT11250.027.49277350
Produced by FastQC (version 0.11.5)