FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493770
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6530.1322478076837394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12700.038.441681
GGTATCA4700.037.3069761
GTGGTAT2250.035.1449621
TCAACGC14200.033.6477664
CAACGCA14350.033.2960475
ATCAACG14350.033.2960473
AACGCAG14800.032.758136
TATCAAC15500.031.2692282
AGAGTAC15450.030.95366511
TACACCG450.00470361430.5546465
ACGCAGA15900.030.5072967
GCAGAGT16450.029.9085879
CGCAGAG16500.029.6094388
CAGAGTA16500.029.19240410
GTAGGTA500.00787000327.4991824
TGGTATC3150.027.2809332
GAGTACG14800.025.5715312
AGTACGG15200.024.67224313
AAGGCGC750.002023490622.93688823
GATTGCG952.3025287E-422.72713567
Produced by FastQC (version 0.11.5)