FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763359
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT22390.1269735771331873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44050.041.3912731
GGTATCA17900.037.6688081
GACGGAC6500.037.57501626
TATCAAC50050.036.343022
TCAACGC49900.036.3144534
CGTATGC6100.035.76901641
ATCAACG51250.035.424973
CTTATAC7050.035.1335641
CAACGCA52200.034.9120065
CTCGTAT5950.034.87174639
AACGCAG52600.034.7118846
TCGTATG6700.033.05536340
TATCTCG6300.032.32283836
TATGCCG6700.032.1048443
ACGCAGA57150.032.067727
CGCAGAG58050.031.5122058
ACGAGAC8100.030.56794222
TATAACG450.00469946330.564474
TATACAC8600.030.386773
CGAGACG8050.029.90511523
Produced by FastQC (version 0.11.5)