FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365211
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG26580.19469517898698444No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT26320.19279071147243906No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG25360.18575883141873306No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22460.16451669375649625No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC19930.1459847598649586No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19850.1453987698604831No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT18990.13909937731237149No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18400.13477770102936468No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGACCA18390.13470445227880526No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTGCTGTCCCACACCTGACAGTAATAGTGAGCCT17700.12965028849020407No Hit
CCCCTGTGCTGGTCATCAATAGGGATAAGAATCGGCCCTCTGGGATCCCA17220.1261343484633511No Hit
GTCCCACACCTGACAGTAATAGTGAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTATTCCC16760.12276490593761696No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16640.12188592093090372No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16510.12093368717363104No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16410.12020119966803668No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15590.1141948021221628No Hit
CAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC15140.11089860834698811No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTG15090.11053236459419094No Hit
CAGTAATAGTGAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGT14960.10958013083691825No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14950.10950688208635881No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14830.10862789707964556No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14510.10628393706174358No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14430.10569794705726807No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14330.10496545955167369No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14180.10386672829328214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)