FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365211
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG23510.17220781256523718No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT23500.17213456381467773No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG23000.16847212628670585No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC22180.162465728740832No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21780.1595357787184545No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTGCTGTCCCACACCTGACAGTAATAGTGAGCCT20140.1475229836267068No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19050.1395388698157281No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT18360.13448470602712695No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGACCA17620.1290642984857286No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17530.12840505973069363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16520.12100693592419047No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15660.11470754337607886No Hit
GTCCCACACCTGACAGTAATAGTGAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTATTCCC15560.1139750558704845No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15500.11353556336712786No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15100.11060561334475037No Hit
CCCCTGTGCTGGTCATCAATAGGGATAAGAATCGGCCCTCTGGGATCCCA14760.1081151558257295No Hit
CAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC14490.10613743956062469No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14430.10569794705726807No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14110.10335398703936607No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTG13950.10218200703041508No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13940.10210875827985563No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13700.10035078826642915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)